Anti-Argonaute 4 with anti-GAPDH internal loading control antibody (ab94772)

Overview

  • Product nameAnti-Argonaute 4 with anti-GAPDH internal loading control antibody
  • Description
    Rabbit polyclonal to Argonaute 4 with anti-GAPDH internal loading control
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: ChIP,Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Argonaute 4 with anti-GAPDH internal loading control.

  • Positive control
    • This cocktail gave a positive signal in both Human Brain and Testis Tissue lysates as well as the following whole cell lysates: HeLa; A431; Jurkat; HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab94772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37, 97 kDa (predicted molecular weight: 37, 97 kDa). ab94772 contains 2 antibodies, generated by raising against an Argonaute 4 immunogen and spiking with anti-GAPDH loading control.
  • Application notesIs unsuitable for ChIP,Flow Cyt,ICC/IF or IHC-P.
  • Target

    • FunctionRequired for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for RNA-directed transcription and replication of the human hapatitis delta virus (HDV).
    • Sequence similaritiesBelongs to the argonaute family. Ago subfamily.
      Contains 1 PAZ domain.
      Contains 1 Piwi domain.
    • Cellular localizationCytoplasm > P-body.
    • Information by UniProt
    • Database links
    • Alternative names
      • AGO4_HUMAN antibody
      • Argonaute4 antibody
      • eIF-2C 4 antibody
      • eIF2C 4 antibody
      • eif2c4 antibody
      • Eukaryotic translation initiation factor 2C 4 antibody
      • hAgo4 antibody
      • Protein argonaute-4 antibody
      see all

    Anti-Argonaute 4 with anti-GAPDH internal loading control antibody images

    • All lanes : Anti-Argonaute 4 with anti-GAPDH internal loading control antibody (ab94772) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Human brain tissue lysate - total protein (ab29466)
      Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 4 : Human testis tissue lysate - total protein (ab30257)
      Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 37, 97 kDa
      Observed band size : 37,97 kDa
      Additional bands at : 52 kDa,60 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 1 minute

    References for Anti-Argonaute 4 with anti-GAPDH internal loading control antibody (ab94772)

    ab94772 has not yet been referenced specifically in any publications.

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