Overview

  • Product nameAnti-ARH antibody
    See all ARH primary antibodies
  • Description
    Goat polyclonal to ARH
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Xenopus laevis
  • Immunogen

    Synthetic peptide: DALKSAGRALIRS, corresponding to amino acids 9-21 of Human ARH.

  • Positive control
    • human liver lysate
  • General notes


    The protein encoded by this gene is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor. Mutations in this gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab5082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40-45 kDa (predicted molecular weight: 35 kDa).Can be blocked with Human ARH peptide (ab23072).

Use RIPA lysis buffer.

Target

  • FunctionAdapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface.
  • Tissue specificityExpressed at high levels in the kidney, liver, and placenta, with lower levels detectable in brain, heart, muscle, colon, spleen, intestine, lung, and leukocytes.
  • Involvement in diseaseDefects in LDLRAP1 are the cause of autosomal recessive hypercholesterolemia (ARH) [MIM:603813]. ARH is a disorder caused by defective internalization of LDL receptors (LDLR) in the liver. ARH has the clinical features of familial hypercholesterolemia (FH) [MIM:143890] homozygotes, including severely elevated plasma LDL cholesterol, tuberous and tendon xanthomata, and premature atherosclerosis. LDL receptor (LDLR) activity measured in skin fibroblasts is normal, as the LDL binding ability.
  • Sequence similaritiesContains 1 PID domain.
  • DomainThe [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif mediates interaction the AP-2 complex subunit AP2B1.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARH antibody
    • ARH GENE antibody
    • ARH_HUMAN antibody
    • ARH1 antibody
    • ARH2 antibody
    • Autosomal recessive hypercholesterolemia protein antibody
    • DKFZp586D0624 antibody
    • FHCB1 antibody
    • FHCB2 antibody
    • LDL receptor adaptor protein antibody
    • Ldlrap1 antibody
    • Low density lipoprotein receptor adapter protein 1 antibody
    • MGC34705 antibody
    • OTTHUMP00000008526 antibody
    see all

Anti-ARH antibody images



  • Predicted band size : 35 kDa


    Goat polyclonal to ARH, ab5082, (3µg/ml) of Human Liver lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

References for Anti-ARH antibody (ab5082)

ab5082 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in IP. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in ret...

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