Overview

  • Product name
  • Description
    Rabbit polyclonal to ARH3
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide corresponding to a region within N terminal amino 93 - 123 acids of Human ARH3.

  • Positive control
    • Hela cell line lysates This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab102979 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 39 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. Poly(ADP-ribose) metabolism may be required for maintenance of the normal function of neuronal cells. Generates ADP-ribose from poly-(ADP-ribose), but does not hydrolyze ADP-ribose-arginine, -cysteine, -diphthamide, or -asparagine bonds.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the ADP-ribosylglycohydrolase family.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • [Protein ADP ribosylarginine] hydrolase like protein 2 antibody
    • [Protein ADP-ribosylarginine] hydrolase-like protein 2 antibody
    • ADP ribosylhydrolase 3 antibody
    • ADP ribosylhydrolase like 2 antibody
    • ADP-ribosylhydrolase 3 antibody
    • Adprhl2 antibody
    • AI836109 antibody
    • ARH3 antibody
    • ARHL2_HUMAN antibody
    • ecto ADP ribosylhydrolase antibody
    • FLJ20446 antibody
    • OTTMUSP00000009757 antibody
    • Poly(ADP ribose) glycohydrolase ARH3 antibody
    • Poly(ADP-ribose) glycohydrolase ARH3 antibody
    • Protein ADP ribosylarginine hydrolase like protein 2 antibody
    • RP23-113P22.3 antibody
    see all

Images

  • Anti-ARH3 antibody (ab102979) at 1/100 dilution + HeLa cell line lysate at 35 µg

    Predicted band size : 39 kDa
  • ab102979 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab102979 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab102979 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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