Overview

  • Product name
    Anti-ARHGAP11A antibody
  • Description
    Rabbit polyclonal to ARHGAP11A
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 973 and 1023 of Human ARHGAP11A using the numbering given in entry NP_055598.1 (GeneID 9824).

  • Positive control
    • HeLa, 293T or NIH3T3 cell lysate. Human skin FFPE tissue sections.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH range: 7 - 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab113261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 114 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Anti-ARHGAP11A antibody images

  • All lanes : Anti-ARHGAP11A antibody (ab113261) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 114 kDa


    Exposure time : 3 minutes
  • Lane 1: 1mg whole cell lysate from HeLa cells was immunoprecipitated with ab113261 at 6µg/mg lysate and 20% IP was loaded on gel.
    Lane 2: same, with a control IgG.
    For WB ab113261 was used at 1µg/ml.
    Detection: chemiluminescence with exposure times of 10 seconds.
  • IHC image of ARHGAP11A staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113261, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-ARHGAP11A antibody (ab113261)

This product has been referenced in:
  • Lawson CD  et al. Rho GTPase Transcriptome Analysis Reveals Oncogenic Roles for Rho GTPase-Activating Proteins in Basal-like Breast Cancers. Cancer Res 76:3826-37 (2016). WB ; Human . Read more (PubMed: 27216196) »
  • Kagawa Y  et al. Cell cycle-dependent Rho GTPase activity dynamically regulates cancer cell motility and invasion in vivo. PLoS One 8:e83629 (2013). WB . Read more (PubMed: 24386239) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab113261.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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