- Datasheet
- Specific References (1)
- Protocols
Overview
- Product nameAnti-ARHGAP17 antibody
- DescriptionRabbit polyclonal to ARHGAP17
- Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide derived from an internal region of human ARHGAP17.
- Positive control
- extracts from LOVO cells
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab74454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa). | |
| ELISA | 1/40000. | |
| ICC/IF | Use at an assay dependent concentration. |
Target
- RelevanceARHGAP17 is a Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. It specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere. RHGAP17 participates in the Ca(2+)-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activator in vitro for RAC1.
- Cellular localizationCytoplasm; Cell membrane (tight junction)
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Database links
- Entrez Gene: 55114 Human
- Entrez Gene: 70497 Mouse
- Entrez Gene: 63994 Rat
- Omim: 608293 Human
- SwissProt: Q68EM7 Human
- SwissProt: Q3UIA2 Mouse
- SwissProt: Q99N37 Rat
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Alternative names
- MST066 antibody
- MST110 antibody
- MSTP038 antibody
see all
Anti-ARHGAP17 antibody images
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Western blot - ARHGAP17 antibody (ab74454)All lanes : Anti-ARHGAP17 antibody (ab74454) at 1/500 dilution
Lane 1 : extracts from LOVO cells
Lane 2 : extracts from LOVO cells with immunizing peptide at 5 µg
Lysates/proteins at 15 µg per lane.
Predicted band size : 95 kDa
Observed band size : 95 kDa -
Immunocytochemistry/ Immunofluorescence - ARHGAP17 antibody (ab74454)This image is courtesy of an anonymous Abreviewab74454 staining ARHGAP17 in Dog MDCK cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde, permeabilzed with 0.5% Triton X-100 and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/200 in PBS containing 0.2% BSA and 5% serum) for 1 hour. A Rhodamine-conjugated goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.
Left-hand panels: Ab74454
Centre panels: anti-myc
Right-hand panels: Merge
MDCK cells transfected with (A) empty vector or (B) vector encolding Myc-tagged human ARHGAP17.
Exogenous human ARHGAP17 was detected but endogenous canine ARHGAP17 was not detected.
References for Anti-ARHGAP17 antibody (ab74454)
This product has been referenced in:
- Nagy Z et al. Cyclic Nucleotide-dependent Protein Kinases Target ARHGAP17 and ARHGEF6 Complexes in Platelets. J Biol Chem 290:29974-83 (2015). Read more (PubMed: 26507661) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"