Customer reviews and FAQs

Overview

  • Product name
    Anti-ARHGAP17 antibody
  • Description
    Rabbit polyclonal to ARHGAP17
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from an internal region of human ARHGAP17.

  • Positive control
    • extracts from LOVO cells

Properties

Applications

Our Abpromise guarantee covers the use of ab74454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
ELISA 1/40000.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    ARHGAP17 is a Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. It specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere. RHGAP17 participates in the Ca(2+)-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activator in vitro for RAC1.
  • Cellular localization
    Cytoplasm; Cell membrane (tight junction)
  • Database links
    • Alternative names
      • MST066 antibody
      • MST110 antibody
      • MSTP038 antibody
      • MSTP066 antibody
      • MSTP110 antibody
      • NADRIN antibody
      • Neuron associated developmentally regulated protein antibody
      • PP367 antibody
      • PP4534 antibody
      • Rho GTPase activating protein 17 antibody
      • Rho type GTPase activating protein 17 antibody
      • RhoGAP interacting with CIP4 homologs 1 antibody
      • RhoGAP interacting with CIP4 homologs protein 1 antibody
      • RICH 1 antibody
      • RICH1 antibody
      • RICH1B antibody
      • WBP15 antibody
      see all

    Anti-ARHGAP17 antibody images

    • Western blot - ARHGAP17 antibody (ab74454)
      Western blot - ARHGAP17 antibody (ab74454)
      All lanes : Anti-ARHGAP17 antibody (ab74454) at 1/500 dilution

      Lane 1 : extracts from LOVO cells
      Lane 2 : extracts from LOVO cells with immunizing peptide at 5 µg

      Lysates/proteins at 15 µg per lane.


      Predicted band size : 95 kDa
      Observed band size : 95 kDa
    • Immunocytochemistry/ Immunofluorescence - ARHGAP17 antibody (ab74454)
      Immunocytochemistry/ Immunofluorescence - ARHGAP17 antibody (ab74454)This image is courtesy of an anonymous Abreview
      ab74454 staining ARHGAP17 in Dog MDCK cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde, permeabilzed with 0.5% Triton X-100 and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/200 in PBS containing 0.2% BSA and 5% serum) for 1 hour. A Rhodamine-conjugated goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.
      Left-hand panels: Ab74454
      Centre panels: anti-myc
      Right-hand panels: Merge
      MDCK cells transfected with (A) empty vector or (B) vector encolding Myc-tagged human ARHGAP17.
      Exogenous human ARHGAP17 was detected but endogenous canine ARHGAP17 was not detected.

      See Abreview

    References for Anti-ARHGAP17 antibody (ab74454)

    This product has been referenced in:
    • Nagy Z  et al. Cyclic Nucleotide-dependent Protein Kinases Target ARHGAP17 and ARHGEF6 Complexes in Platelets. J Biol Chem 290:29974-83 (2015). Read more (PubMed: 26507661) »

    See 1 Publication for this product

    Publishing research using ab74454? Please let us know so that we can cite the reference in this datasheet.

    Product Wall

    Submit an abreview for ab74454 Submit a question for ab74454

    2 Abreviews or Q&A

    10
    Votes: Highest First

    I would like to know whether the antigen used to raise this antibody is sufficiently similar to a zebrafish antigen. The full length of the orthologous Arhgap17 is shown below.

    Unfortunately, there is not significant homology (53%), therefore ab74454 will not likely react with Zebrafish. There is maximum of 2 aa in a row which are similar.

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ARHGAP17 antibody

     Good
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Dog Cell (MDCK cells)
    Specification
    MDCK cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5 % Triton X-100
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More
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    Verified customer

    Submitted Apr 28 2010

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