Overview

  • Product name
  • Description
    Mouse polyclonal to ARHGAP20
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    ARHGAP20 (NP_065860, 1 a.a. ~ 1191 a.a) human protein.

  • Positive control
    • Rat brain tissue lysate, ARHGAP20 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 132 kDa).

Target

  • Function
    GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.
  • Tissue specificity
    Expressed predominantly in the brain. Lower expression is found in lymph nodes.
  • Involvement in disease
    Note=A chromosomal aberration involving ARHGAP20 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(X;11)(q21;q23) with BRWD3 does not result in fusion transcripts but disrupts both genes.
  • Sequence similarities
    Contains 1 PH domain.
    Contains 1 Ras-associating domain.
    Contains 1 Rho-GAP domain.
  • Developmental stage
    Low expression is found in fetal liver.
  • Information by UniProt
  • Database links
  • Alternative names
    • Arhgap20 antibody
    • KIAA1391 antibody
    • RARHOGAP antibody
    • RHG20_HUMAN antibody
    • Rho GTPase activating protein 20 antibody
    • Rho GTPase-activating protein 20 antibody
    • Rho type GTPase activating protein 20 antibody
    • Rho-type GTPase-activating protein 20 antibody
    see all

Anti-ARHGAP20 antibody images

  • Anti-ARHGAP20 antibody (ab68918) at 1/500 dilution + rat brain lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 132 kDa
    Observed band size : 132 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-ARHGAP20 antibody (ab68918) at 1/500 dilution

    Lane 1 : transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 132 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)

References for Anti-ARHGAP20 antibody (ab68918)

ab68918 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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