Overview

  • Product name
    Anti-ARHGAP31 antibody
  • Description
    Rabbit polyclonal to ARHGAP31
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 1163-1261 (QDLDIVAHAL TGRRNSAPVS VSAVRTSFMV KMCQARAVPV IPPKIQYTQI PQPLPSQSSG ENGVQPLERS QEGPSSTSGT TQKPAKDDSP SSLESSKEE) of Human ARHGAP31.

  • Positive control
    • Human placenta tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab122331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Functions as a GTPase-activating protein (GAP) for RAC1 and CDC42. Required for cell spreading, polarized lamellipodia formation and cell migration.
  • Sequence similarities
    Contains 1 Rho-GAP domain.
  • Developmental stage
    Mainly expressed in fetal heart and muscle.
  • Post-translational
    modifications
    Phosphorylation on Thr-789 reduces GAP activity.
  • Cellular localization
    Cell projection > lamellipodium. Cell junction > focal adhesion.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHGAP31 antibody
    • Cdc42 GTPase-activating protein antibody
    • RHG31_HUMAN antibody
    • Rho GTPase-activating protein 31 antibody

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, cytoplasm and golgi apparatus. Recommended concentration of ab122331 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122331, at 1/300 dilution, staining ARHGAP31 in paraffin-embedded Human placenta tissue, by Immunohistochemistry.

References

ab122331 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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