Overview

  • Product nameAnti-ARHGAP4 antibody
    See all ARHGAP4 primary antibodies
  • Description
    Mouse polyclonal to ARHGAP4
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human ARHGAP4 protein (AAH52303).

  • Positive control
    • Human placenta tissue lysate. Jurkat cell lysate. ARHGAP4 transfected 293T cell lysate. ARHGAP4 expressing 293 cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab89077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 105 kDa.
Flow Cyt Use at an assay dependent concentration. ab37355-Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceARHGAP4 (Rho GTPase-activating protein 4) has an inhibitory effect on stress fiber organization. It is a member of the group of signaling proteins involved in regulation of the small GTP-binding proteins of the RAS superfamily.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • ARHGAP 4 antibody
    • C1 antibody
    • GTPase-activating protein, Rho, 4 antibody
    • KIAA0131 antibody
    • OTTHUMP00000026004 antibody
    • p115 antibody
    • RGC 1 antibody
    • RGC1 antibody
    • Rho GAP hematopoietic protein C1 antibody
    • Rho GAP hematopoietic protein C1 antibody
    • Rho GTPase activating protein 4 antibody
    • Rho type GTPase activating protein 4 antibody
    • RhoGAP 4 antibody
    • RhoGAP4 antibody
    • SrGAP4 antibody
    see all

Anti-ARHGAP4 antibody images

  • Anti-ARHGAP4 antibody (ab89077) at 1/500 dilution + human placenta tissue lysate at 50 µg

    Predicted band size : 105 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 115 kDa. We are unsure as to the identity of these extra bands.
  • Anti-ARHGAP4 antibody (ab89077) at 1/500 dilution + Jurkat cell lysate at 50 µg

    Predicted band size : 105 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-ARHGAP4 antibody (ab89077) at 1/500 dilution

    Lane 1 : ARHGAP4 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 105 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

References for Anti-ARHGAP4 antibody (ab89077)

ab89077 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"