Overview

  • Product name
  • Description
    Rabbit polyclonal to ARHGAP42
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: DPSIPLPQPQ SRSGSRRTRA ICLSTGSRKP RGRYTPCLAE PDSDSYSSSP DSTPMGSIES LSSHSSEQNS TTKSASCQ corresponding to amino acids in Human ARHGAP42.

  • Positive control
    • Human testis tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May act as a GTPase activator.
  • Sequence similarities
    Contains 1 BAR domain.
    Contains 1 PH domain.
    Contains 1 Rho-GAP domain.
    Contains 1 SH3 domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHGAP42 antibody
    • FLJ32810 antibody
    • GRAF3 antibody
    • RHG42_HUMAN antibody
    • Rho GTPase-activating protein 10-like antibody
    • Rho GTPase-activating protein 42 antibody
    • Rho-type GTPase-activating protein 42 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab122602 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122602, at 1/50 dilution, staining ARHGAP42 in paraffin embedded Human testis tissue by Immunohistochemistry.

References

ab122602 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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