Overview

  • Product nameAnti-ARHGEF1 antibody
    See all ARHGEF1 primary antibodies
  • Description
    Mouse monoclonal to ARHGEF1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: CRPGPEGQLA ATALRKVLSL KQLLFPAEED NGAGPPRDGD GVPGGGPLSP ARTQEIQENL LSLEETMKQL EELEEEFCRL RPLLSQLGGN SVPQPGCT, corresponding to amino acids 830-927 of Human ARHGEF1

Properties

Applications

Our Abpromise guarantee covers the use of ab56462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSeems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.
    • Tissue specificityUbiquitously expressed.
    • Sequence similaritiesContains 1 DH (DBL-homology) domain.
      Contains 1 PH domain.
      Contains 1 RGSL (RGS-like) domain.
    • DomainThe RGSL domain, also known as rgRGS domain, is necessary but not sufficient for GAP activity.
      The DH domain is involved in interaction with CCPG1.
    • Post-translational
      modifications
      Phosphorylated by PKCA. Angiotensin-2 induced Tyr-738 phosphorylation is mediated by JAK2.
    • Cellular localizationCytoplasm. Membrane. Translocated to the membrane by activated GNA13 or LPA stimulation.
    • Information by UniProt
    • Database links
    • Alternative names
      • 115 kD protein antibody
      • 115 kDa guanine nucleotide exchange factor antibody
      • ARHG1_HUMAN antibody
      • ARHGEF 1 antibody
      • ARHGEF1 antibody
      • GEF 1 antibody
      • GEF1 antibody
      • LBCL 2 antibody
      • LBCL2 antibody
      • LSC antibody
      • Lsc homolog antibody
      • p115 RhoGEF antibody
      • p115-RhoGEF antibody
      • p115RhoGEF antibody
      • Rho guanine nucleotide exchange factor (GEF) 1 antibody
      • Rho guanine nucleotide exchange factor 1 antibody
      • Sub1.5 antibody
      see all

    Anti-ARHGEF1 antibody images

    • Western blot against tagged recombinant protein immunogen using ab56462 ARHGEF1 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    References for Anti-ARHGEF1 antibody (ab56462)

    ab56462 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab56462.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"