Anti-ARHGEF10 antibody (ab110009)

Overview

  • Product nameAnti-ARHGEF10 antibody
    See all ARHGEF10 primary antibodies
  • Description
    Rabbit polyclonal to ARHGEF10
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthesized peptide derived from internal sequence of Human ARHGEF10.

  • Positive control
    • Human breast carcinoma tissue COS7 cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab110009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 152 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMay play a role in developmental myelination of peripheral nerves.
  • Involvement in diseaseDefects in ARHGEF10 are the cause of slowed nerve conduction velocity (SNCV) [MIM:608236]. Affected individuals present a reduction in nerve conduction velocities without any clinical signs of peripheral or central nervous system dysfunction. SNCV inheritance is autosomal dominant.
  • Sequence similaritiesContains 1 DH (DBL-homology) domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHGA_HUMAN antibody
    • ARHGEF10 antibody
    • DKFZp686H0726 antibody
    • GEF10 antibody
    • KIAA0294 antibody
    • MGC131664 antibody
    • Rho guanine nucleotide exchange factor (GEF) 10 antibody
    • Rho guanine nucleotide exchange factor 10 antibody
    see all

Anti-ARHGEF10 antibody images

  • Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using ab110009.
  • All lanes : Anti-ARHGEF10 antibody (ab110009) at 1/500 dilution

    Lane 1 : COS7 cells
    Lane 2 : COS7 cells treated with the synthesized peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 152 kDa

References for Anti-ARHGEF10 antibody (ab110009)

ab110009 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Rat Cell lysate - whole cell (Cortical Neurons))
Loading amount 35 µg
Specification Rat Cell lysate - whole cell (Cortical Neurons)
Treatment Lentivirus knocking down(shRNA) and overexpression (OE) endogenous ARHGEF10
Gel Running Conditions Reduced Denaturing (8%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Nov 14 2012

DISCOUNT CODE: * - For ab110009
* - *
* - For ab134220

Expiration date: */, for all 3 codes

I am very pleased to hear you would like to accept our offer and test ab110009/ab117825
/ab134220 in rat samples. This code will g...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"