Overview

  • Product name
  • Description
    Rabbit polyclonal to ARHGEF10
  • Tested applications
    Suitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    CGGQGHRRVHRKAR

    conjugated to KLH, corresponding to amino acids 1335-1348 of Human ARHGEF10

  • Positive control
    • Human Brain cerebellum tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab64170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1 - 1/1000.
IHC-P Use a concentration of 20 µg/ml.

Target

  • Function
    May play a role in developmental myelination of peripheral nerves.
  • Involvement in disease
    Defects in ARHGEF10 are the cause of slowed nerve conduction velocity (SNCV) [MIM:608236]. Affected individuals present a reduction in nerve conduction velocities without any clinical signs of peripheral or central nervous system dysfunction. SNCV inheritance is autosomal dominant.
  • Sequence similarities
    Contains 1 DH (DBL-homology) domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHGA_HUMAN antibody
    • ARHGEF10 antibody
    • DKFZp686H0726 antibody
    • GEF10 antibody
    • KIAA0294 antibody
    • MGC131664 antibody
    • Rho guanine nucleotide exchange factor (GEF) 10 antibody
    • Rho guanine nucleotide exchange factor 10 antibody
    see all

Images

  • ab64170 at 20µg/ml staining ARHGEF10 in human brain cerebellum by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

References

ab64170 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Cortical Neurons)
Loading amount
15 µg
Specification
Cortical Neurons
Treatment
Lentivirus knocking down endogenous ARHGEF10
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

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Verified customer

Submitted Sep 16 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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