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Rabbit polyclonal to ARHGEF3
Predicted to work with:
Mouse, Rat, Chicken
Recombinant fragment, corresponding to a region within amino acids 81-337 of Human ARHGEF3 (Uniprot ID: Q9NR81).
NT2D1 and MCF7 whole cell lysate; MCF7 cells; Human hepatoma tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 60 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/1000.
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. ARHGEF3 encodes a guanine nucleotide exchange factor, which specifically activates two members of the Rho GTPase family: RHOA and RHOB, both of which have a role in bone cell biology. It has been identified that genetic variation in ARHGEF3 plays a role in the determination of bone mineral density (BMD), suggesting a role in postmenopausal osteoporosis.
59.8 kDA protein antibody
Western blot - Anti-ARHGEF3 antibody (ab154263)
Anti-ARHGEF3 antibody (ab154263) at 1/2000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size:
7.5% SDS PAGE
Blocking 5% non-fat milk
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGEF3 antibody (ab154263)
Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue labeling ARHGEF3 with ab154263 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-ARHGEF3 antibody (ab154263)
Immunofluorescent analysis of methanol-fixed MCF7 cells labeling ARHGEF3 with ab154263 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"