Overview

  • Product name
  • Description
    Rabbit polyclonal to ARHGEF3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 81-337 of Human ARHGEF3 (Uniprot ID: Q9NR81).

  • Positive control
    • NT2D1 and MCF7 whole cell lysate; MCF7 cells; Human hepatoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 60 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance
    Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. ARHGEF3 encodes a guanine nucleotide exchange factor, which specifically activates two members of the Rho GTPase family: RHOA and RHOB, both of which have a role in bone cell biology. It has been identified that genetic variation in ARHGEF3 plays a role in the determination of bone mineral density (BMD), suggesting a role in postmenopausal osteoporosis.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • 1200004I24Rik antibody
    • 59.8 kDA protein antibody
    • 9830169H03Rik antibody
    • ARHGEF 3 antibody
    • C76747 antibody
    • DKFZP434F2429 antibody
    • Exchange factor found in platelets and leukemic and neuronal tissues antibody
    • Exchange factor found in platelets and leukemic and neuronal tissues XPLN antibody
    • FLJ98126 antibody
    • GEF 3 antibody
    • GEF3 antibody
    • MGC118905 antibody
    • Rho guanine nucleotide exchange factor (GEF) 3 antibody
    • Rho guanine nucleotide exchange factor 3 antibody
    • RhoGEF protein antibody
    • STA 3 antibody
    • STA3 antibody
    • XPLN antibody
    see all

Images

  • Anti-ARHGEF3 antibody (ab154263) at 1/2000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size: 60 kDa



    7.5% SDS PAGE

    Blocking 5% non-fat milk

  • Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue labeling ARHGEF3 with ab154263 at 1/500 dilution.
  • Immunofluorescent analysis of methanol-fixed MCF7 cells labeling ARHGEF3 with ab154263 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

References

ab154263 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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