The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: Antibody detection limit dilution 1:16,000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 177 kDa. Approx 170kDa band observed in Human HeLa lysates
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Tissue specificityMainly expressed in kidney, liver, pancreas, lung and placenta.