The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 5-15µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 87 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Acts as a RAC1 guanine nucleotide exchange factor (GEF).
Involvement in disease
Defects in ARHGEF6 are the cause of mental retardation X-linked type 46 (MRX46) [MIM:300436]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
ab91562 at 1 µg/ml detecting ARHGEF6 in Jurkat whole cell lysate by WB following IP. Lane 1; IP with ab91562 at 10 µg/mg of lysate. Lane 2; IP with an antibody which recognizes an downstream epitope of ARHGEF6. Lane 3; control IgG. In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded. Detection utilised ECL with a 10 second exposure.
Western blot - Anti-ARHGEF6 antibody (ab91562)
All lanes : Anti-ARHGEF6 antibody (ab91562) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg Lane 2 : Jurkat whole cell lysate at 15 µg Lane 3 : Jurkat whole cell lysate at 5 µg