Overview

  • Product name
  • Description
    Rabbit polyclonal to ARHGEF6
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 50-100 (PGSVEKFCLD PQTEADCINN INDFLKGCAT LQVEIFDPDD LYSGVNFSKV L) of Human ARHGEF6 (NP_004831.1).

  • Positive control
    • Jurkat whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antibody was affinity purified using an epitope specific to ARHGEF6 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 5-15µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 87 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Acts as a RAC1 guanine nucleotide exchange factor (GEF).
    • Tissue specificity
      Ubiquitous.
    • Involvement in disease
      Defects in ARHGEF6 are the cause of mental retardation X-linked type 46 (MRX46) [MIM:300436]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
    • Sequence similarities
      Contains 1 CH (calponin-homology) domain.
      Contains 1 DH (DBL-homology) domain.
      Contains 1 PH domain.
      Contains 1 SH3 domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha Pix antibody
      • Alpha-Pix antibody
      • ARHG6_HUMAN antibody
      • ARHGEF6 antibody
      • COOL 2 antibody
      • COOL-2 antibody
      • COOL2 antibody
      • Mental retardation, X linked 46 antibody
      • MRX46 antibody
      • PAK interacting exchange factor alpha antibody
      • PAK-interacting exchange factor alpha antibody
      • PIXA antibody
      • Rac/Cdc42 guanine exchange factor (GEF) 6 antibody
      • Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 antibody
      • Rac/Cdc42 guanine nucleotide exchange factor 6 antibody
      • Rho guanine nucleotide exchange factor 6 antibody
      see all

    Images

    • ab91562 at 1 µg/ml detecting ARHGEF6 in Jurkat whole cell lysate by WB following IP. Lane 1; IP with ab91562 at 10 µg/mg of lysate. Lane 2; IP with an antibody which recognizes an downstream epitope of ARHGEF6. Lane 3; control IgG. In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded. Detection utilised ECL with a 10 second exposure.
    • All lanes : Anti-ARHGEF6 antibody (ab91562) at 0.1 µg/ml

      Lane 1 : Jurkat whole cell lysate at 50 µg
      Lane 2 : Jurkat whole cell lysate at 15 µg
      Lane 3 : Jurkat whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 87 kDa


      Exposure time : 3 minutes

    References

    This product has been referenced in:
    • Yu J  et al. Wnt5a induces ROR1/ROR2 heterooligomerization to enhance leukemia chemotaxis and proliferation. J Clin Invest 126:585-98 (2016). Read more (PubMed: 26690702) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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