Overview

  • Product name
  • Description
    Rabbit polyclonal to ARHGEF9
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human, Orangutan
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human ARHGEF9.

    (Peptide available as ab98294.)

  • Positive control
    • This antibody gave a positive signal in human brain and heart tissues lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).

Target

  • Function
    Acts as guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters.
  • Tissue specificity
    Detected in brain. Detected at low levels in heart.
  • Involvement in disease
    Defects in ARHGEF9 are a cause of startle disease with epilepsy (STHEE) [MIM:300607]; also known as hyperekplexia with epilepsy. Startle disease is a genetically heterogeneous neurologic disorder. STHE is characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile stimuli.
  • Sequence similarities
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9630036L12Rik antibody
    • A230067K14 antibody
    • ARHG9_HUMAN antibody
    • Arhgef9 antibody
    • Cdc42 guanine nucleotide exchange factor (GEF) 9 antibody
    • Collybistin antibody
    • collybistin I antibody
    • EIEE8 antibody
    • HPEM 2 antibody
    • hPEM-2 collybistin antibody
    • KIAA0424 antibody
    • mKIAA0424 antibody
    • OTTHUMP00000023424 antibody
    • OTTHUMP00000023425 antibody
    • OTTHUMP00000023427 antibody
    • PEM 2 antibody
    • PEM 2 homolog antibody
    • PEM-2 homolog antibody
    • PEM2 antibody
    • Rac/Cdc42 guanine nucleotide exchange factor 9 antibody
    • Rho guanine nucleotide exchange factor 9 antibody
    • RP11 357C3.4 antibody
    • RP23 230K20.3 antibody
    see all

Anti-ARHGEF9 antibody images

  • All lanes : Anti-ARHGEF9 antibody (ab87148) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 61 kDa


    Exposure time : 12 minutes

References for Anti-ARHGEF9 antibody (ab87148)

ab87148 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E13.5 mouse embryo (heart))
Permeabilization
No
Specification
E13.5 mouse embryo (heart)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Paraformaldehyde
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Submitted Feb 02 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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