Overview

  • Product name
    Anti-ARHGEF9 antibody - C-terminal
    See all ARHGEF9 primary antibodies
  • Description
    Rabbit polyclonal to ARHGEF9 - C-terminal
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the C-terminal region of human ARHGEF9 conjugated to KLH

  • Positive control
    • 293 cell lysates transiently transfected with the ARHGEF9 gene. Human brain tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab71350 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/10 - 1/50.
WB 1/50 - 1/100. Detects a band of approximately 55 kDa (predicted molecular weight: 61 kDa).

Target

  • Function
    Acts as guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters.
  • Tissue specificity
    Detected in brain. Detected at low levels in heart.
  • Involvement in disease
    Defects in ARHGEF9 are a cause of startle disease with epilepsy (STHEE) [MIM:300607]; also known as hyperekplexia with epilepsy. Startle disease is a genetically heterogeneous neurologic disorder. STHE is characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile stimuli.
  • Sequence similarities
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9630036L12Rik antibody
    • A230067K14 antibody
    • ARHG9_HUMAN antibody
    • Arhgef9 antibody
    • Cdc42 guanine nucleotide exchange factor (GEF) 9 antibody
    • Collybistin antibody
    • collybistin I antibody
    • EIEE8 antibody
    • HPEM 2 antibody
    • hPEM-2 collybistin antibody
    • KIAA0424 antibody
    • mKIAA0424 antibody
    • OTTHUMP00000023424 antibody
    • OTTHUMP00000023425 antibody
    • OTTHUMP00000023427 antibody
    • PEM 2 antibody
    • PEM 2 homolog antibody
    • PEM-2 homolog antibody
    • PEM2 antibody
    • Rac/Cdc42 guanine nucleotide exchange factor 9 antibody
    • Rho guanine nucleotide exchange factor 9 antibody
    • RP11 357C3.4 antibody
    • RP23 230K20.3 antibody
    see all

Images

  • All lanes : Anti-ARHGEF9 antibody - C-terminal (ab71350) at 1/50 dilution

    Lane 1 : Nontransfected 293 cell lysates
    Lane 2 : 293 cell lysates transiently transfected with the ARHGEF9 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 61 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa (possible non-specific binding).
  • ab71350 at 1/10 dilution, staining ARHGEF9 in human brain by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

References

This product has been referenced in:
  • Simon S  et al. Inter-kingdom Signaling by the Legionella Quorum Sensing Molecule LAI-1 Modulates Cell Migration through an IQGAP1-Cdc42-ARHGEF9-Dependent Pathway. PLoS Pathog 11:e1005307 (2015). Read more (PubMed: 26633832) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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