Overview

  • Product name
  • Description
    Rabbit polyclonal to ARHI
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein of Human ARHI (NP_004666.1).

  • Positive control
    • WB: WiDr cell line lysates IHC-P: Human brain tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab107051 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab107051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 26 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificity
    Expressed in normal ovarian and breast epithelial cells but not in ovarian and breast cancers.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Di-Ras family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DIRA3 antibody
    • DIRA3_HUMAN antibody
    • DIRAS family GTP binding RAS like 3 antibody
    • DIRAS family GTPase 3 antibody
    • DIRAS family, GTP-binding RAS-like protein 3 antibody
    • DIRAS3 antibody
    • Distinct subgroup of the Ras family member 3 antibody
    • GTP binding protein Di Ras3 antibody
    • GTP-binding protein Di-Ras3 antibody
    • NOEY2 antibody
    • Ras homolog gene family member I antibody
    • Rho related GTP binding protein RhoI antibody
    • Rho-related GTP-binding protein RhoI antibody
    • RHOI antibody
    see all

Images

  • ab107051 flow cytometric analysis of WiDr cells (right histogram) compared to a hegative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Anti-ARHI antibody (ab107051) at 1/100 dilution + WiDr cell line lysates at 35 µg

    Predicted band size: 26 kDa

  • ab107051, at a1/50 dilution, staining ARHI in formalin fixed and paraffin embedded Human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

This product has been referenced in:
  • Ouyang J  et al. The role of aplysia ras homolog I in colon cancer cell invasion and adhesion. Exp Ther Med 14:5193-5199 (2017). Read more (PubMed: 29201236) »
  • Li LL  et al. JMJD2A contributes to breast cancer progression through transcriptional repression of the tumor suppressor ARHI. Breast Cancer Res 16:R56 (2014). Read more (PubMed: 24886710) »

See all 2 Publications for this product

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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