Recombinant
RabMAb

Anti-ARID1A antibody [EPR13501-73] (ab182561)

Overview

  • Product name
    Anti-ARID1A antibody [EPR13501-73]
    See all ARID1A primary antibodies
  • Description
    Rabbit monoclonal [EPR13501-73] to ARID1A
  • Tested applications
    Suitable for: ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human ARID1A aa 1200-1350. The exact sequence is proprietary.
    Database link: O14497

  • Positive control
    • Human kidney, Human adenocarcinoma of endometrium without ARIDIA mutation; SH-SY5Y cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab182561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
IHC-P 1/1000.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function
    Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Binds DNA non-specifically. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
  • Tissue specificity
    Highly expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and PBL, and at a much lower level in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
  • Sequence similarities
    Contains 1 ARID domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • actin-dependent regulator of chromatin subfamily F member 1 antibody
    • ARI1A_HUMAN antibody
    • ARID domain containing protein 1A antibody
    • ARID domain-containing protein 1A antibody
    • ARID1A antibody
    • AT rich interactive domain 1A (SWI like) antibody
    • AT rich interactive domain 1A antibody
    • AT rich interactive domain containing protein 1A antibody
    • AT-rich interactive domain-containing protein 1A antibody
    • B120 antibody
    • BAF250 antibody
    • BAF250A antibody
    • BM029 antibody
    • brain protein 120 antibody
    • BRG1 associated factor 250 antibody
    • BRG1 associated factor 250a antibody
    • BRG1-associated factor 250 antibody
    • BRG1-associated factor 250a antibody
    • C1ORF4 antibody
    • chromatin remodeling factor p250 antibody
    • chromosome 1 open reading frame 4 antibody
    • ELD antibody
    • hELD antibody
    • hOSA1 antibody
    • matrix-associated antibody
    • MRD14 antibody
    • Osa homolog 1 antibody
    • OSA1 antibody
    • OSA1 nuclear protein antibody
    • P270 antibody
    • SMARCF1 antibody
    • SWI like protein antibody
    • SWI SNF complex protein p270 antibody
    • SWI-like protein antibody
    • SWI/SNF complex protein p270 antibody
    • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily f, member 1 antibody
    • SWI/SNF-related antibody
    see all

Images

  • Immunofluorescent analysis of SH-SY5Y cells labeling ARID1A with ab182561 at 1/500 and Goat anti rabbit IgG(Alexa Fluor®555) at 1/200. Image at the right stained with DAPI.

  • Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling ARID1A with purified ab92512 at 1/500 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue  (left image) labeling ARID1A using ab182561 at 1/1000 dilution. Right image: human clear cell carcinoma of kidney with ARID1A mutation. A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of endometrium without ARID1A mutation (left image) labeling ARID1A using ab182561 at 1/1000 dilution. Right image: human adenocarcinoma of endometrium with ARID1A mutation. A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.

References

ab182561 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

These two antibodies are extremely similar. They recognise the same protein in the same applications and species however they are different clones. The immunogen used for both antibodies is the same (aa1200-1350).

Although we have not mapped t...

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