Anti-ARID2 antibody [2036C1a] (ab51019)

Overview

  • Product nameAnti-ARID2 antibody [2036C1a]
    See all ARID2 primary antibodies
  • Description
    Mouse monoclonal [2036C1a] to ARID2
  • Tested applicationsSuitable for: WB, ICC, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment

  • Positive control
    • HeLa cells. HEK293 whole cell lysate. NIH3T3 whole cell lysate. F2408 whole cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane.
  • ClonalityMonoclonal
  • Clone number2036C1a
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Detects a band of approximately 240 kDa (predicted molecular weight: 197 kDa).
ICC 1/5.
Dot blot Use at an assay dependent dilution.

Target

  • FunctionInvolved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). May be involved in targeting the complex to different genes. May be involved in regulating transcriptional activation of cardiac genes.
  • Tissue specificityHighly expressed in heart and testis.
  • Sequence similaritiesContains 1 ARID domain.
    Contains 1 C2H2-type zinc finger.
    Contains 1 RFX-type winged-helix DNA-binding domain.
  • Developmental stageUp-regulated in adult heart (at protein level).
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARID domain containing protein 2 antibody
    • ARID domain-containing protein 2 antibody
    • ARID2 antibody
    • ARID2_HUMAN antibody
    • AT rich interactive domain 2 (ARID, RFX like) antibody
    • AT rich interactive domain containing protein 2 antibody
    • AT-rich interactive domain-containing protein 2 antibody
    • BAF antibody
    • BAF200 antibody
    • BRG1 associated factor 200 antibody
    • BRG1-associated factor 200 antibody
    • DKFZp686G052 antibody
    • DKFZp779P0222 antibody
    • FLJ30619 antibody
    • KIAA1557 antibody
    • p200 antibody
    • Zinc finger protein with activation potential antibody
    • zip zap antibody
    • zipzap antibody
    • Zipzap/p200 antibody
    see all

Anti-ARID2 antibody [2036C1a] images

  • Anti-ARID2 antibody [2036C1a] (ab51019) at 1/50 dilution + HEK293 whole cell lysate at 25 µg

    Secondary
    Mouse IgG anibody at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 197 kDa
    Observed band size : 240 kDa (why is the actual band size different from the predicted?)
  • HeLa cells stained with ab51019 at 1/5 dilution, followed by Alexa Fluor 488 Goat Anti-Mouse IgG at 1/200 dilution.
  • Anti-ARID2 antibody [2036C1a] (ab51019) at 1/50 dilution + NIH3T3 whole cell lysate at 25 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 197 kDa
    Observed band size : 240 kDa (why is the actual band size different from the predicted?)
  • Anti-ARID2 antibody [2036C1a] (ab51019) at 1/50 dilution + F2408 whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 197 kDa
    Observed band size : 240 kDa (why is the actual band size different from the predicted?)

References for Anti-ARID2 antibody [2036C1a] (ab51019)

ab51019 has not yet been referenced specifically in any publications.

Product Wall

Thank you for forwarding the details of your protocol. The protocol looks fine, especially since you were able to detect a different protein of a similar size. I have credited your original order.

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