Overview

  • Product nameAnti-ARID2 antibody
    See all ARID2 primary antibodies
  • Description
    Rabbit polyclonal to ARID2
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Bat
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 1785-1835 of Human ARID2 (NP_689854.2).

  • Positive control
    • Human Breast Carcinoma, Human Prostate Carcinoma, and Human Testicular Seminoma tissues This antibody gave a positive result when used in the following methanol fixed cell lines: MCF-7.
  • General notes

    Concentration is optimized for IHC and not determined

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab113283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
ICC/IF 1/200.

Target

  • FunctionInvolved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). May be involved in targeting the complex to different genes. May be involved in regulating transcriptional activation of cardiac genes.
  • Tissue specificityHighly expressed in heart and testis.
  • Sequence similaritiesContains 1 ARID domain.
    Contains 1 C2H2-type zinc finger.
    Contains 1 RFX-type winged-helix DNA-binding domain.
  • Developmental stageUp-regulated in adult heart (at protein level).
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARID domain containing protein 2 antibody
    • ARID domain-containing protein 2 antibody
    • ARID2 antibody
    • ARID2_HUMAN antibody
    • AT rich interactive domain 2 (ARID, RFX like) antibody
    • AT rich interactive domain containing protein 2 antibody
    • AT-rich interactive domain-containing protein 2 antibody
    • BAF antibody
    • BAF200 antibody
    • BRG1 associated factor 200 antibody
    • BRG1-associated factor 200 antibody
    • DKFZp686G052 antibody
    • DKFZp779P0222 antibody
    • FLJ30619 antibody
    • KIAA1557 antibody
    • p200 antibody
    • Zinc finger protein with activation potential antibody
    • zip zap antibody
    • zipzap antibody
    • Zipzap/p200 antibody
    see all

Anti-ARID2 antibody images

  • Staining of Human ARID2 in a Formalin/PFA-fixed paraffin-embedded section of Human Testicular Seminoma using ab113283 at a dilution of 1/250.
  • ab113283 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab113283 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-ARID2 antibody (ab113283)

ab113283 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"