Overview

  • Product name
  • Description
    Rabbit polyclonal to ARID5A
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: MAAGLMHFPP TSFDSALRHR LCPASSAWHA PPVTTYAAPH FFHLNTKL, corresponding to C terminal amino acids 547-594 of Human ARID5A (NP_997646).

  • Positive control
    • 721_B cell lysate IF/ICC: HeLa cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab81149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 22, 34, 64 kDa (predicted molecular weight: 64 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/62500.

IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Relevance
    ARID5A is a member of the ARID protein family, that have diverse functions but all appear to play important roles in development, tissue-specific gene expression, and regulation of cell growth.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • ARID domain containing protein 5A antibody
    • ARID-containing protein 5A antibody
    • AT rich interactive domain 5A antibody
    • AT rich interactive domain 5A (MRF1 like) antibody
    • AT rich interactive domain containing protein 5A antibody
    • Modulator recognition factor 1 antibody
    • MRF 1 antibody
    • MRF1 antibody
    • RFVG5814 antibody
    • RP11 363D14 antibody
    see all

Images

  • ab81149 stained HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81149 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-ARID5A antibody (ab81149) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 64 kDa
    Observed band size : 64 kDa
    Additional bands at : 34 kDa,<22 kDa. We are unsure as to the identity of these extra bands.Gel concentration 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling ARID5A with ab81149 at 5.0µg/ml.

References

This product has been referenced in:
  • Nyati KK  et al. TLR4-induced NF-?B and MAPK signaling regulate the IL-6 mRNA stabilizing protein Arid5a. Nucleic Acids Res 45:2687-2703 (2017). WB . Read more (PubMed: 28168301) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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