The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 64 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Titre using peptide based assay: 1/62500.
RelevanceARID5A is a member of the ARID protein family, that have diverse functions but all appear to play important roles in development, tissue-specific gene expression, and regulation of cell growth.