Overview

  • Product nameAnti-ARID5A antibody
    See all ARID5A primary antibodies
  • Description
    Rabbit polyclonal to ARID5A
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide within amino acids 108-157 of Human ARID5A: RRLWKNVYDE LGGSPGSTSA ATCTRRHYER LVLPYVRHLK GEDDKPLPTS

  • Positive control
    • 721_B cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab81207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 64 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

  • RelevanceARID5A is a member of the ARID protein family, that have diverse functions but all appear to play important roles in development, tissue-specific gene expression, and regulation of cell growth.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • ARID domain containing protein 5A antibody
    • ARID-containing protein 5A antibody
    • AT rich interactive domain 5A antibody
    • AT rich interactive domain 5A (MRF1 like) antibody
    • AT rich interactive domain containing protein 5A antibody
    • Modulator recognition factor 1 antibody
    • MRF 1 antibody
    • MRF1 antibody
    • RFVG5814 antibody
    • RP11 363D14 antibody
    see all

Anti-ARID5A antibody images

  • Anti-ARID5A antibody (ab81207) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 64 kDa
    Observed band size : 64 kDa

References for Anti-ARID5A antibody (ab81207)

ab81207 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Mouse Cell lysate - whole cell (HEK 293 T cell line)
Specification HEK 293 T cell line
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 24 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"