Overview

  • Product nameAnti-ARIH1 antibody
    See all ARIH1 primary antibodies
  • Description
    Goat polyclonal to ARIH1
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:

    HEGYEKDLWEYIED

    by a Cysteine residue linker, corresponding to C terminal amino acids 544-557 of Human ARIH1

  • Positive control
    • Human Adrenal tissue; U937 lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab115300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 64 kDa.
ELISA 1/32000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMight act as an E3 ubiquitin-protein ligase, or as part of the E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the RBR family. Ariadne subfamily.
    Contains 1 IBR-type zinc finger.
    Contains 2 RING-type zinc fingers.
  • Cellular localizationCytoplasm. Mainly cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARI 1 antibody
    • ARI antibody
    • ARI-1 antibody
    • ARI1 antibody
    • ARI1_HUMAN antibody
    • Ariadne Drosophila homolog of antibody
    • Ariadne homolog antibody
    • Ariadne homolog ubiquitin conjugating enzyme E2 binding protein 1 antibody
    • Ariadne ubiquitin conjugating enzyme E2 binding protein homolog 1 antibody
    • ARIH 1 antibody
    • ARIH1 antibody
    • E3 ubiquitin protein ligase ARIH1 antibody
    • H7 AP2 antibody
    • H7-AP2 antibody
    • HARI antibody
    • HHARI antibody
    • human homologue of ariadne antibody
    • Monocyte protein 6 antibody
    • MOP 6 antibody
    • MOP-6 antibody
    • MOP6 antibody
    • Protein ariadne 1 homolog antibody
    • Protein ariadne-1 homolog antibody
    • Protein ariadne1 homolog antibody
    • UbcH7 binding protein antibody
    • UBCH7 BP antibody
    • UbcH7-binding protein antibody
    • UBCH7BP antibody
    • UbcM4 interacting protein antibody
    • UbcM4-interacting protein antibody
    • Ubiquitin conjugating enzyme E2 binding protein 1 antibody
    • Ubiquitin-conjugating enzyme E2-binding protein 1 antibody
    see all

Anti-ARIH1 antibody images

  • ab115300, at 5 µg/ml, staining ARIH1 in formalin-fixed, paraffin-embedded Human Adrenal tissue by Immunohistochemistry, using a biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-ARIH1 antibody (ab115300) at 0.5 µg/ml + U937 lysate (in RIPA buffer) at 30 µg
    Developed using the ECL technique

    Predicted band size : 64 kDa

References for Anti-ARIH1 antibody (ab115300)

ab115300 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"