The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 64 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionMight act as an E3 ubiquitin-protein ligase, or as part of the E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the RBR family. Ariadne subfamily. Contains 1 IBR-type zinc finger. Contains 2 RING-type zinc fingers.
ab115300, at 5 µg/ml, staining ARIH1 in formalin-fixed, paraffin-embedded Human Adrenal tissue by Immunohistochemistry, using a biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-ARIH1 antibody (ab115300)
Anti-ARIH1 antibody (ab115300) at 0.5 µg/ml + U937 lysate (in RIPA buffer) at 30 µg Developed using the ECL technique
Predicted band size : 64 kDa
References for Anti-ARIH1 antibody (ab115300)
has not yet been referenced specifically in any publications.
Publishing research using ab115300? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"