Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab3891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWestern Blot: Approx 65 kDa band seen in U937 lysate [Predicted MW of approx. 61kDa according to NP_005735].
    Recommended for use at 0.2-1.0µg/ml.
    Not tested in other applications.

    Optimal working dilutions / concentrations should be determined by the end user.
  • Target

    • FunctionMight act as an E3 ubiquitin-protein ligase, or as part of the E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates.
    • Tissue specificityWidely expressed.
    • Sequence similaritiesBelongs to the RBR family. Ariadne subfamily.
      Contains 1 IBR-type zinc finger.
      Contains 2 RING-type zinc fingers.
    • Cellular localizationCytoplasm. Mainly cytoplasmic.
    • Information by UniProt
    • Database links
    • Alternative names
      • ARI 1 antibody
      • ARI antibody
      • ARI-1 antibody
      • ARI1 antibody
      • ARI1_HUMAN antibody
      • Ariadne Drosophila homolog of antibody
      • Ariadne homolog antibody
      • Ariadne homolog ubiquitin conjugating enzyme E2 binding protein 1 antibody
      • Ariadne ubiquitin conjugating enzyme E2 binding protein homolog 1 antibody
      • ARIH 1 antibody
      • ARIH1 antibody
      • E3 ubiquitin protein ligase ARIH1 antibody
      • H7 AP2 antibody
      • H7-AP2 antibody
      • HARI antibody
      • HHARI antibody
      • human homologue of ariadne antibody
      • Monocyte protein 6 antibody
      • MOP 6 antibody
      • MOP-6 antibody
      • MOP6 antibody
      • Protein ariadne 1 homolog antibody
      • Protein ariadne-1 homolog antibody
      • Protein ariadne1 homolog antibody
      • UbcH7 binding protein antibody
      • UBCH7 BP antibody
      • UbcH7-binding protein antibody
      • UBCH7BP antibody
      • UbcM4 interacting protein antibody
      • UbcM4-interacting protein antibody
      • Ubiquitin conjugating enzyme E2 binding protein 1 antibody
      • Ubiquitin-conjugating enzyme E2-binding protein 1 antibody
      see all

    Anti-ARIH1 antibody images

    • All lanes : Anti-ARIH1 antibody (ab3891) at 1/1500 dilution

      Lane 1 : NIH3T3 whole cell lysate at 30 µg
      Lane 2 : NIH3T3 whole cell lysate at 30 µg
      Lane 3 : Partially purified NIH3T3 protein mixture at 10 µg

      Secondary
      Alkaline Phosphatase-conjugated anti-goat IgG polyclonal at 1/15000 dilution

      Performed under reducing conditions.

      Observed band size : 65 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 45 kDa (possible non-specific binding),50 kDa (possible non-specific binding).

      Exposure time : 15 minutes

      This image is courtesy of an Abreview submitted by T Lawson

      Lanes 1 and 2 - 12% SDS-PAGE.

      Lane 3 - 4-15% SDS-PAGE.

      Lanes 1 and 3 - developed with colorimetric substrate.

      Lane 2 - developed with ECF+.

      See Abreview

    • ab3891 staining (0.5µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ab3891 staining (0.5µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    References for Anti-ARIH1 antibody (ab3891)

    This product has been referenced in:
    • Wong ES  et al. Relative sensitivity of parkin and other cysteine-containing enzymes to stress-induced solubility alterations. J Biol Chem 282:12310-8 (2007). Read more (PubMed: 17329252) »
    • LaVoie MJ  et al. The effects of oxidative stress on parkin and other E3 ligases. J Neurochem 103:2354-68 (2007). Read more (PubMed: 17883392) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (12%)
    Sample Mouse Cell lysate - whole cell (NIH3T3 cells)
    Specification NIH3T3 cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Username

    Dr. T Lawson

    Verified customer

    Submitted Sep 16 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"