Overview

  • Product name
  • Description
    Goat polyclonal to ARIH2
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:

    EQRRRTLLKDFHDT

    , corresponding to C terminal amino acids 480-493 of Human ARIH2.

Properties

Applications

Our Abpromise guarantee covers the use of ab17014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml.

Approx 60kDa band observed in nuclear lysates of Hela and K562 (Calculated MW of 57.8kDa according to NP_006312.1). Customer's experiments gave a band at approx 60kDa in lysates in both Human HeLa and Mouse Muscle (IM2 & C2C12) cells. This band disappeared after RNAi knock-down.

ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function
    E3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. May play a role in myelopoiesis.
  • Tissue specificity
    Widely expressed with higher expression in granulocytes.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the RBR family. Ariadne subfamily.
    Contains 1 IBR-type zinc finger.
    Contains 2 RING-type zinc fingers.
  • Domain
    RING-type 1 and RING-type 2 are required for the inhibitory function in myelopoiesis.
  • Post-translational
    modifications
    Ubiquitinated. Ubiquitination promotes proteasomal degradation.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • HT005 antibody
    • all trans retinoic acid inducible RING finger antibody
    • Ari 2 antibody
    • Ari-2 antibody
    • ARI2 antibody
    • ARI2_HUMAN antibody
    • Ariadne 2 antibody
    • Ariadne 2 protein homolog antibody
    • ariadne homolog 2 (Drosophila) antibody
    • ariadne homolog 2 antibody
    • Ariadne RBR E3 ubiquitin protein ligase 2 antibody
    • ARIH 2 antibody
    • Arih2 antibody
    • E3 ubiquitin protein ligase ARIH2 antibody
    • E3 ubiquitin-protein ligase ARIH2 antibody
    • protein ariadne 2 homolog antibody
    • Protein ariadne-2 homolog antibody
    • Triad 1 antibody
    • TRIAD1 antibody
    • Triad1 protein antibody
    • UIP48 antibody
    see all

Images

  • All lanes : Anti-ARIH2 antibody (ab17014) at 2 µg/ml

    Lane 1 : HeLa nuclear lysate
    Lane 2 : K562 nuclear lysate

    Lysates/proteins at 35 µg per lane.

References

ab17014 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab17014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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