Overview

  • Product nameAnti-ARK5 antibody
    See all ARK5 primary antibodies
  • Description
    Rabbit polyclonal to ARK5
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Hamster, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to sequence from within N terminal amino acids 1-100 of Human ARK5.

  • Positive control
    • Mouse brain tissue lysate, Human breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab37641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • FunctionInvolved in tolerance to glucose starvation. Phosphorylates ATM. Suppresses Fas-induced apoptosis by phosphorylation of CASP6, thus suppressing the activation of the caspase and the subsequent cleavage of CFLAR.
  • Tissue specificityExpressed at high levels in heart and brain, and at lower levels in skeletal muscle, kidney, ovary, placenta, lung and liver.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMP activated protein kinase family member 5 antibody
    • AMPK related protein kinase 5 antibody
    • AMPK-related protein kinase 5 antibody
    • NUAK family SNF1 like kinase antibody
    • NUAK family SNF1-like kinase 1 antibody
    • Nuak1 antibody
    • NUAK1_HUMAN antibody
    • Omphalocele kinase 1 antibody
    • Probable serine/threonine protein kinase KIAA0537 antibody
    see all

Anti-ARK5 antibody images

  • Anti-ARK5 antibody (ab37641) at 1/100 dilution + Mouse brain tissue lysate

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
  • ab37641, at 1/50 dilution, staining ARK5 in Human breast carcinoma by Immunohistochemistry, Formalin fixed, Paraffin embedded tissue. ab37641 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

References for Anti-ARK5 antibody (ab37641)

ab37641 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Blocking step Odyssey block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
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Submitted Apr 24 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"