Overview

  • Product nameAnti-ARK5 antibody
    See all ARK5 primary antibodies
  • Description
    Rabbit polyclonal to ARK5
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from the C-terminal region of human ARK5 conjugated to KLH

  • Positive control
    • Mouse brain tissue lysate and human breast carcinoma tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab71814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).

Target

  • FunctionInvolved in tolerance to glucose starvation. Phosphorylates ATM. Suppresses Fas-induced apoptosis by phosphorylation of CASP6, thus suppressing the activation of the caspase and the subsequent cleavage of CFLAR.
  • Tissue specificityExpressed at high levels in heart and brain, and at lower levels in skeletal muscle, kidney, ovary, placenta, lung and liver.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMP activated protein kinase family member 5 antibody
    • AMPK related protein kinase 5 antibody
    • AMPK-related protein kinase 5 antibody
    • NUAK family SNF1 like kinase antibody
    • NUAK family SNF1-like kinase 1 antibody
    • Nuak1 antibody
    • NUAK1_HUMAN antibody
    • Omphalocele kinase 1 antibody
    • Probable serine/threonine protein kinase KIAA0537 antibody
    see all

Anti-ARK5 antibody images

  • Anti-ARK5 antibody (ab71814) at 1/100 dilution + mouse brain tissue lysate at 12.5 µg

    Secondary
    HRP-anti-rabbit
    Developed using the ECL technique

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
    Additional bands at : 17 kDa,40 kDa. We are unsure as to the identity of these extra bands.
  • ab71814 at 1/50 dilution, staining ARK5 in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

References for Anti-ARK5 antibody (ab71814)

ab71814 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"