Recombinant
RabMAb

Anti-ARL 1 antibody [EPR10595] (ab155982)

Overview

  • Product name
    Anti-ARL 1 antibody [EPR10595]
  • Description
    Rabbit monoclonal [EPR10595] to ARL 1
  • Tested applications
    Suitable for: WB, IHC-P, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human ARL 1 (UniProt: P40616).

  • Positive control
    • HepG2, MCF7, HeLa and K562 cell lysate; Human liver and pancreas tissues. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: CaCo2
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10595
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab155982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      GTP-binding protein that has very low efficiency as allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Can activate phospholipase D with very low efficiency. Important for normal function of the Golgi apparatus.
    • Tissue specificity
      Detected in heart, liver, lung and liver (at protein level). Detected in fetal heart, lung, liver and kidney. Detected in adult heart, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • Sequence similarities
      Belongs to the small GTPase superfamily. Arf family.
    • Cellular localization
      Golgi apparatus membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADP ribosylation factor like 1 antibody
      • ADP-ribosylation factor-like protein 1 antibody
      • ARFL1 antibody
      • Arl1 antibody
      • ARL1_HUMAN antibody
      see all

    Images

    • ICC/IF image of ab155982 stained CaCo2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab155982 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • ab155982 showing +ve staining in Human normal prostate.
    • ab155982 showing +ve staining in Human gastric adenocarcinoma.
    • ab155982 showing +ve staining in Human endometrial carcinoma.
    • ab155982 showing +ve staining in Human normal brain.
    • ab155982 showing +ve staining in Human normal liver.
    • All lanes : Anti-ARL 1 antibody [EPR10595] (ab155982) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labeled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 20 kDa
    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ARL 1 with ab155982 at 1/50 dilution.
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling ARL 1 with ab155982 at 1/50 dilution

    References

    ab155982 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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