Overview

  • Product name
  • Description
    Rabbit polyclonal to ARL1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow, Pig, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human ARL1.

    (Peptide available as ab88216.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab76156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
ICC/IF 1/500. Use with paraformaldehyde or methanol fixed cells.

Target

  • Function
    GTP-binding protein that has very low efficiency as allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Can activate phospholipase D with very low efficiency. Important for normal function of the Golgi apparatus.
  • Tissue specificity
    Detected in heart, liver, lung and liver (at protein level). Detected in fetal heart, lung, liver and kidney. Detected in adult heart, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Arf family.
  • Cellular localization
    Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP ribosylation factor like 1 antibody
    • ADP-ribosylation factor-like protein 1 antibody
    • ARFL1 antibody
    • Arl1 antibody
    • ARL1_HUMAN antibody
    see all

Anti-ARL1 antibody images

  • All lanes : Anti-ARL1 antibody (ab76156) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 20 kDa
    Observed band size : 20 kDa
    Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • ab76156 (1/500) staining ARL1 in assynchronous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.

    See Abreview

References for Anti-ARL1 antibody (ab76156)

This product has been referenced in:
  • Eiseler T  et al. Protein Kinase D2 Assembles a Multiprotein Complex at the Trans-Golgi Network to Regulate Matrix Metalloproteinase Secretion. J Biol Chem 291:462-77 (2016). Read more (PubMed: 26507660) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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