Overview

  • Product name
  • Description
    Rabbit polyclonal to ARL2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within the C terminal amino acids 123-184 of Human ARL2.

  • Positive control
    • H1299 whole cell lysate, Human Breast, Human Small Intestine

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 1% BSA, PBS, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 21 kDa.
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function
    Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Regulates formation of new microtubules and centrosome integrity. Prevents the TBCD-induced microtubule destruction. Participates in association with TBCD, in the disassembly of the apical junction complexes. Antagonizes the effect of TBCD on epithelial cell detachment and tight and adherens junctions disassembly. Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. Component of a regulated secretory pathway involved in Ca(2+)-dependent release of acetylcholine. Required for normal progress through the cell cycle.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Arf family.
  • Post-translational
    modifications
    Not N-myristoylated.
  • Cellular localization
    Mitochondrion intermembrane space. Cytoplasm > cytoskeleton > centrosome. Nucleus. Cytoplasm. The complex formed with ARL2BP, ARL2 and SLC25A6 is expressed in mitochondria. The complex formed with ARL2BP, ARL2 and SLC25A4 is expressed in mitochondria (By similarity). Not detected in the Golgi, nucleus and on the mitotic spindle. Centrosome-associated throughout the cell cycle. Not detected to interphase microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP ribosylation factor like 2 antibody
    • ADP-ribosylation factor-like protein 2 antibody
    • ARFL2 antibody
    • Arl184 antibody
    • Arl2 antibody
    • ARL2_HUMAN antibody
    see all

Anti-ARL2 antibody images

  • Anti-ARL2 antibody (ab109742) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 21 kDa
  • ab109742 at 10 µg/ml staining ARL2 in Human breast by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
  • ab109742 at 10 µg/ml staining ARL2 in Human small intestine by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

References for Anti-ARL2 antibody (ab109742)

ab109742 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Nov 05 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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