Overview

  • Product name
    Anti-ARL2 antibody - N-terminal
    See all ARL2 primary antibodies
  • Description
    Rabbit polyclonal to ARL2 - N-terminal
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Positive control
    • Hela cell line lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab71288 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

Target

  • Function
    Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Regulates formation of new microtubules and centrosome integrity. Prevents the TBCD-induced microtubule destruction. Participates in association with TBCD, in the disassembly of the apical junction complexes. Antagonizes the effect of TBCD on epithelial cell detachment and tight and adherens junctions disassembly. Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. Component of a regulated secretory pathway involved in Ca(2+)-dependent release of acetylcholine. Required for normal progress through the cell cycle.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Arf family.
  • Post-translational
    modifications
    Not N-myristoylated.
  • Cellular localization
    Mitochondrion intermembrane space. Cytoplasm > cytoskeleton > centrosome. Nucleus. Cytoplasm. The complex formed with ARL2BP, ARL2 and SLC25A6 is expressed in mitochondria. The complex formed with ARL2BP, ARL2 and SLC25A4 is expressed in mitochondria (By similarity). Not detected in the Golgi, nucleus and on the mitotic spindle. Centrosome-associated throughout the cell cycle. Not detected to interphase microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP ribosylation factor like 2 antibody
    • ADP-ribosylation factor-like protein 2 antibody
    • ARFL2 antibody
    • Arl184 antibody
    • Arl2 antibody
    • ARL2_HUMAN antibody
    see all

Images

  • Anti-ARL2 antibody - N-terminal (ab71288) at 1/100 dilution + Hela cell line lysate at 35 µg/ml

    Predicted band size : 21 kDa
    Observed band size : 21 kDa
    Additional bands at : 32 kDa,56 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Price HP  et al. The small GTPase ARL2 is required for cytokinesis in Trypanosoma brucei. Mol Biochem Parasitol 173:123-31 (2010). Read more (PubMed: 20653091) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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