Overview

  • Product nameAnti-ARL3 antibody
    See all ARL3 primary antibodies
  • Description
    Rabbit polyclonal to ARL3
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (10-30 amino acids in length) conjugated to KLH, from the C terminal of last 50 amino acids of Human ARL3.

  • Positive control
    • Human breast carcinoma, A2058 cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab38860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/60. Detects a band of approximately 24 kDa (predicted molecular weight: 20 kDa).
IHC-P 1/50 - 1/100.

Target

  • FunctionSmall GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for normal cytokinesis and cilia signaling.
  • Tissue specificityExpressed in the retina. Strongly expressed in connecting cilium, the myoid region of the inner segments (IS) and in cone photoreceptors (at protein level).
  • Sequence similaritiesBelongs to the small GTPase superfamily. Arf family.
  • Cellular localizationGolgi apparatus membrane. Cytoplasm > cytoskeleton > spindle. Nucleus. Cytoplasm > cytoskeleton > centrosome. Cytoplasm. Cell projection > cilium. Detected predominantly in the photoreceptor connecting cilium. Present on the mitotic spindle. Centrosome-associated throughout the cell cycle. Not detected to interphase microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP ribosylation factor like 3 antibody
    • ADP-ribosylation factor-like protein 3 antibody
    • Arf like 3 antibody
    • Arf like protein 3 antibody
    • ARFL3 antibody
    • ARL3 antibody
    • ARL3_HUMAN antibody
    see all

Anti-ARL3 antibody images

  • Formalin fixed and paraffin embedded human breast carcinoma tissue stained with ab38860, followed by AEC staining. ab38860 at a 1/50 dilution.
  • Anti-ARL3 antibody (ab38860) at 1/60 dilution + A2058 cell line lysate at 35 µg

    Predicted band size : 20 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa,35 kDa,40 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ARL3 antibody (ab38860)

ab38860 has not yet been referenced specifically in any publications.

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