Overview

  • Product name
    Anti-ARL4C antibody
  • Description
    Rabbit polyclonal to ARL4C
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence corresponding to C terminal amino acids 88-192 of Human ARL4C.

  • Positive control
    • Human skeletal muscle tissue.
      RT-4 and U-251 MG cell lysate.
      A-431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab122025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function
    Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. May be involved in transport between a perinuclear compartment and the plasma membrane, apparently linked to the ABCA1-mediated cholesterol secretion pathway. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in the GDP-bound form. Regulates the microtubule-dependent intracellular vesicular transport from early endosome to recycling endosome process.
  • Tissue specificity
    Expressed in several tumor cell lines (at protein level). Expressed in lung, brain, leukocytes and placenta.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Arf family.
  • Cellular localization
    Cell projection > filopodium. Cell membrane. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP ribosylation factor like 4C antibody
    • ADP ribosylation factor like 7 antibody
    • ADP-ribosylation factor-like protein 4C antibody
    • ADP-ribosylation factor-like protein 7 antibody
    • ADP-ribosylation factor-like protein LAK antibody
    • Arl4c antibody
    • ARL4C_HUMAN antibody
    • ARL7 antibody
    • LAK antibody
    see all

Images

  • ab122025 at 1/120 dilution staining ARL4C in paraffin-embedded Human skeletal muscle by Immunohistochemistry.
  • All lanes : Anti-ARL4C antibody (ab122025) at 1/250 dilution

    Lane 1 : RT-4 cell lysate.
    Lane 2 : U-251 MG cell lysate.
    Lane 3 : Human plasma
    Lane 4 : Human Liver tissue lysate.
    Lane 5 : Human Tonsil tissue lysate.

    Developed using the ECL technique
  • ab122025 at 1 µg/ml staining ARL4C (green) in PFA-fixed, Triton X-100 permeabilized, A-431 cells by Immunofluorescence.

References

ab122025 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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