Anti-ARL5B antibody - C-terminal (ab170810)

Overview

  • Product name
    Anti-ARL5B antibody - C-terminal
  • Description
    Rabbit polyclonal to ARL5B - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein within Human ARL5B aa 129-161 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q96KC2

  • Positive control
    • CEM cells and cell lysate; Human kidney tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    ab170810 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 20 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Binds and exchanges GTP and GDP.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Arf family.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP ribosylation factor like 5B antibody
    • ADP ribosylation factor like 8 antibody
    • ADP ribosylation like factor 8 antibody
    • ADP-ribosylation factor-like protein 5B antibody
    • ADP-ribosylation factor-like protein 8 antibody
    • Arl5b antibody
    • ARL5B_HUMAN antibody
    • ARL8 antibody
    • OTTHUMP00000019260 antibody
    see all

Images

  • Flow cytometric analysis of CEM cells labeling ARL5B with ab170810 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human kidney tissue labeling ARL5B with ab170810 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-ARL5B antibody - C-terminal (ab170810) at 1/50 dilution + CEM cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 20 kDa

References

ab170810 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab170810.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up