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Rabbit polyclonal to ARL6IP6
antigen sequence corresponding to amino acids 1-92 (MSFAESGWRS ALRRRGPGTP GPVARPSYSS FTQGDSWGEG EVDEEEGCDQ VARDLRAEFS AGAWSEPRKR SVLPPDGNGS PVLPDKRNGI FP) of Human ARL6IP6.
Human duodenum tissue; ARL6IP6 transfected HEK293T cell over-expression lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
Belongs to the ARL6IP6 family.
Information by UniProt
ADP ribosylation like factor 6 interacting protein 6 antibody
ADP-ribosylation factor-like protein 6-interacting protein 6 antibody
Immunocytochemistry/ Immunofluorescence - Anti-ARL6IP6 antibody (ab121448)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nuclear membrane, cytoplasm and centrosome. Recommended concentration of ab121448 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL6IP6 antibody (ab121448)
ab121448, at 1/225 dilution, staining ARL6IP6 in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
Western blot - Anti-ARL6IP6 antibody (ab121448)
All lanes : Anti-ARL6IP6 antibody (ab121448) at 1/250 dilution Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2 : Negative control (vector only transfected HEK293T lysate) Lane 3 : ARL6IP6 transfected HEK293T over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)) Developed using the ECL technique.
has not yet been referenced specifically in any publications.
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