Overview

  • Product name
    Anti-ARL6IP6 antibody
  • Description
    Rabbit polyclonal to ARL6IP6
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence corresponding to amino acids 1-92 (MSFAESGWRS ALRRRGPGTP GPVARPSYSS FTQGDSWGEG EVDEEEGCDQ VARDLRAEFS AGAWSEPRKR SVLPPDGNGS PVLPDKRNGI FP) of Human ARL6IP6.

  • Positive control
    • Human duodenum tissue; ARL6IP6 transfected HEK293T cell over-expression lysate

Properties

  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Sequence similarities
    Belongs to the ARL6IP6 family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP ribosylation like factor 6 interacting protein 6 antibody
    • ADP-ribosylation factor-like protein 6-interacting protein 6 antibody
    • Aip-6 antibody
    • AR6P6_HUMAN antibody
    • ARL-6-interacting protein 6 antibody
    • ARL6IP6 antibody
    • PFAAP1 antibody
    • Phosphonoformate immuno-associated protein 1 antibody
    • Regulated by phosphonoformate antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nuclear membrane, cytoplasm and centrosome. Recommended concentration of ab121448 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121448, at 1/225 dilution, staining ARL6IP6 in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
  • All lanes : Anti-ARL6IP6 antibody (ab121448) at 1/250 dilution

    Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
    Lane 2 : Negative control (vector only transfected HEK293T lysate)
    Lane 3 : ARL6IP6 transfected HEK293T over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))

    Developed using the ECL technique

References

ab121448 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab121448.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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