Anti-NS21 antibody (ab235416)
Key features and details
- Rabbit polyclonal to NS21
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NS21 antibody -
Description
Rabbit polyclonal to NS21 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan -
Immunogen
Recombinant fragment corresponding to Human NS21 aa 400-700.
Database link: Q7Z3E5 -
Positive control
- WB: Jurkat, CEM and A549 whole cell lysates. IHC-P: Human tonsil tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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WB |
1/500 - 1/2000. Predicted molecular weight: 92 kDa.
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Notes |
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IHC-P
1/20 - 1/200. |
WB
1/500 - 1/2000. Predicted molecular weight: 92 kDa. |
Target
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Tissue specificity
Strongly expressed in most melanomas and melanocytes. Weakly expressed in the testis. -
Sequence similarities
Contains 1 LisH domain. - Information by UniProt
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Database links
- Entrez Gene: 536462 Cow
- Entrez Gene: 80210 Human
- Entrez Gene: 78795 Mouse
- Entrez Gene: 100173624 Orangutan
- Entrez Gene: 301579 Rat
- GenBank: NM_025139 Human
- GenBank: NP_079415 Human
- SwissProt: Q2KI89 Cow
see all -
Alternative names
- ARM antibody
- armadillo repeat containing 9 antibody
- armadillo/beta-catenin-like repeats antibody
see all
Images
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All lanes : Anti-NS21 antibody (ab235416) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : CEM whole cell lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 92 kDa -
Paraffin-embedded human tonsil tissue stained for NS21 using ab235416 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab235416 has not yet been referenced specifically in any publications.