Anti-ARMET/ARP antibody (ab126321)
Key features and details
- Rabbit polyclonal to ARMET/ARP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-ARMET/ARP antibody
See all ARMET/ARP primary antibodies -
Description
Rabbit polyclonal to ARMET/ARP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster -
Immunogen
Synthetic peptide corresponding to Human ARMET/ARP aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab154773) -
Positive control
- This antibody gave a positive signal in both HepG2 and Jurkat whole cell lysates. IHC-P: Human cerebral cortex FFPE tissue sections This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 20 kDa).
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 20 kDa). |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. -
Sequence similarities
Belongs to the ARMET family. -
Domain
The N-terminal region may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response. -
Post-translational
modificationsMay contain sialic acid residues. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 540432 Cow
- Entrez Gene: 7873 Human
- Entrez Gene: 74840 Mouse
- Entrez Gene: 315989 Rat
- Omim: 601916 Human
- SwissProt: P80513 Cow
- SwissProt: P55145 Human
- SwissProt: Q9CXI5 Mouse
see all -
Alternative names
- Arginine rich mutated in early stage tumors antibody
- Arginine rich protein antibody
- Arginine-rich protein antibody
see all
Images
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ab126321 staining ARMET/ARP in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab126321 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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All lanes : Anti-ARMET/ARP antibody (ab126321) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Additional bands at: 135 kDa, 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutesThe band observed at 17 kDa could potentially be a cleaved form of ARMET/ARP due to the presence of a 24 amino acid signal peptide. This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab126321 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
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IHC image of ARMET/ARP staining in human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab126321, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab126321 has been referenced in 2 publications.
- Wang CH et al. Activator protein-1 is a novel regulator of mesencephalic astrocyte-derived neurotrophic factor transcription. Mol Med Rep 18:5765-5774 (2018). PubMed: 30365109
- Lynch JM et al. Binding of a glaucoma-associated myocilin variant to the aB-crystallin chaperone impedes protein clearance in trabecular meshwork cells. J Biol Chem 293:20137-20156 (2018). PubMed: 30389787