Anti-ARMET/ARP antibody (ab67271)
Key features and details
- Rabbit polyclonal to ARMET/ARP
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-ARMET/ARP antibody
See all ARMET/ARP primary antibodies -
Description
Rabbit polyclonal to ARMET/ARP -
Host species
Rabbit -
Specificity
ab67271 does not crossreact with CDNF. -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human ARMET/ARP (C terminal). The immunogen is located within the last 50 amino acids. 12 amino acid synthetic peptide.
Database link: P55145 -
Positive control
- WB: HEK-293, A431, A549, CaCo-2, Daudi, HeLa, Jurkat, SK-N-SH, THP-1, NIH/3T3, and YB2/0 cell lysates. IHC-P: Human brain tissue.
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General notes
Please see ab253243 as alternative product for testing human samples in WB and ICC/IF.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 20 µg/ml.
Not validated in mouse and rat samples |
|
IHC-P |
Use a concentration of 2.5 µg/ml.
Not validated in mouse and rat samples |
|
WB |
Use a concentration of 0.125 - 2 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 21 kDa).
Blocking conditions: 5% non-fat milk in TBS at 4 overnight. |
Notes |
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ICC/IF
Use a concentration of 20 µg/ml. Not validated in mouse and rat samples |
IHC-P
Use a concentration of 2.5 µg/ml. Not validated in mouse and rat samples |
WB
Use a concentration of 0.125 - 2 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 21 kDa). Blocking conditions: 5% non-fat milk in TBS at 4 overnight. |
Target
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Function
Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. -
Sequence similarities
Belongs to the ARMET family. -
Domain
The N-terminal region may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response. -
Post-translational
modificationsMay contain sialic acid residues. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 7873 Human
- Entrez Gene: 74840 Mouse
- Entrez Gene: 315989 Rat
- Omim: 601916 Human
- SwissProt: P55145 Human
- SwissProt: Q9CXI5 Mouse
- SwissProt: P0C5H9 Rat
- Unigene: 436446 Human
see all -
Alternative names
- Arginine rich mutated in early stage tumors antibody
- Arginine rich protein antibody
- Arginine-rich protein antibody
see all
Images
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All lanes : Anti-ARMET/ARP antibody (ab67271) at 2 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 3 : A549 (human lung carcinoma cell line) cell lysate
Lane 4 : CaCo-2 (human colorectal adenocarcinoma cell line) cell lysate
Lane 5 : Daudi (human Burkitt's lymphoma cell line) cell lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 7 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 8 : SK-N-SH (human neuroblastoma cell line) cell lysate
Lane 9 : THP-1 (human monocytic leukemia cell line) cell lysate
Lane 10 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 11 : YB2/0 (rat B cell lymphoblast cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?Blocking conditions: 5% non-fat milk in TBS at 4 overnight.
Primary antibody: Room temperature for 1 hour
Diluting buffer: 5% NFDM/TBST.
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ab67271 at 2.5 μg/ml staining of ARMET/ARP in human brain tissue using IHC-P. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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All lanes : Anti-ARMET/ARP antibody (ab67271) at 1 µg/ml
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ARMET/ARP knock-out HEK293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?Diluting buffer: 5% NFDM/TBST.
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ab67271 at 2.5 μg/ml staining of ARMET/ARP in human brain tissue using IHC-P. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescent analysis of 4% paraformaldehydefixed human brain cells labeling ARMET/ARP in Human Brain cells using ab67271 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab67271 has been referenced in 25 publications.
- Hou X et al. Mesencephalic astrocyte-derived neurotrophic factor reprograms macrophages to ameliorate acetaminophen-induced acute liver injury via p38 MAPK pathway. Cell Death Dis 13:100 (2022). PubMed: 35110525
- Tejedor G et al. MANF Produced by MRL Mouse-Derived Mesenchymal Stem Cells Is Pro-regenerative and Protects From Osteoarthritis. Front Cell Dev Biol 9:579951 (2021). PubMed: 33738280
- Liu XC et al. Increased MANF Expression in the Inferior Temporal Gyrus in Patients With Alzheimer Disease. Front Aging Neurosci 13:639318 (2021). PubMed: 33994992
- de Seny D et al. New Proteins Contributing to Immune Cell Infiltration and Pannus Formation of Synovial Membrane from Arthritis Diseases. Int J Mol Sci 23:N/A (2021). PubMed: 35008858
- He M et al. Mesencephalic astrocyte-derived neurotrophic factor ameliorates steatosis in HepG2 cells by regulating hepatic lipid metabolism. World J Gastroenterol 26:1029-1041 (2020). PubMed: 32205994