Anti-Aromatase antibody (ab115271)

Overview

  • Product nameAnti-Aromatase antibody
    See all Aromatase primary antibodies
  • Description
    Goat polyclonal to Aromatase
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cynomolgus Monkey
  • Immunogen

    Synthetic peptide:

    C-HDLSLHPDETKN

    , corresponding to internal sequence amino acids 475-486 of Human Aromatase (AAA52132.1).

  • Positive control
    • Human placenta lysate; Human placenta tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab115271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 58 kDa.
ELISA 1/128000.
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionCatalyzes the formation of aromatic C18 estrogens from C19 androgens.
  • Tissue specificityBrain, placenta and gonads.
  • Involvement in diseaseDefects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
    Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries.
  • Sequence similaritiesBelongs to the cytochrome P450 family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARO antibody
    • ARO1 antibody
    • Aromatase antibody
    • CP19A_HUMAN antibody
    • CPV1 antibody
    • CYAR antibody
    • CYP19 antibody
    • Cyp19a1 antibody
    • CYPXIX antibody
    • Cytochrome P-450AROM antibody
    • Cytochrome P450 19A1 antibody
    • Cytochrome P450, family 19, subfamily A, polypeptide 1 antibody
    • Cytochrome P450, subfamily XIX (aromatization of androgens) antibody
    • Estrogen synthase antibody
    • Estrogen synthetase antibody
    • Flavoprotein linked monooxygenase antibody
    • MGC104309 antibody
    • Microsomal monooxygenase antibody
    • OTTHUMP00000162543 antibody
    • OTTHUMP00000198350 antibody
    • P 450AROM antibody
    see all

Anti-Aromatase antibody images

  • Anti-Aromatase antibody (ab115271) at 0.1 µg/ml + Human placenta lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 58 kDa
  • ab115271, at 4 ug/ml, staining Aromatase in formalin fixed, paraffin embedded Human placenta tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated anti-goat IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References for Anti-Aromatase antibody (ab115271)

ab115271 has not yet been referenced specifically in any publications.

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Thank you for your enquiry regarding ab115271.

Currently, no published reference is available regarding this antibody. As soon as we have further information, we will update our on-line document accordingly. There is an IHC image on the on-li...

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