Anti-Aromatase antibody (ab18995)
Key features and details
- Rabbit polyclonal to Aromatase
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Aromatase antibody
See all Aromatase primary antibodies -
Description
Rabbit polyclonal to Aromatase -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chicken, Cow, Pig -
Immunogen
Synthetic peptide surrounding amino acid 385 of human Aromatase (Peptide available as ab51924.)
-
General notes
This product is manufactured by BioVision, an Abcam company and was previously called 3599 Aromatase Antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (3) |
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa). An additional 35 kDa band can also be detected in Jurkat cells (we are unsure of the identity).
|
Notes |
---|
WB
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa). An additional 35 kDa band can also be detected in Jurkat cells (we are unsure of the identity). |
Target
-
Function
Catalyzes the formation of aromatic C18 estrogens from C19 androgens. -
Tissue specificity
Brain, placenta and gonads. -
Involvement in disease
Defects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 414854 Chicken
- Entrez Gene: 1588 Human
- Omim: 107910 Human
- SwissProt: P19098 Chicken
- SwissProt: P11511 Human
- Unigene: 260074 Human
-
Alternative names
- ARO antibody
- ARO1 antibody
- Aromatase antibody
see all
Images
-
Anti-Aromatase antibody (ab18995) at 1 µg/ml + Human brain normal tissue lysate - membrane extract (ab29456) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Datasheets and documents
-
SDS download
-
Datasheet download
References (67)
ab18995 has been referenced in 67 publications.
- Krawczyk K et al. Persistent organic pollutants affect steroidogenic and apoptotic activities in granulosa cells and reactive oxygen species concentrations in oocytes in the mouse. Reprod Fertil Dev 35:294-305 (2023). PubMed: 36403477
- Zhang Y et al. Zika Virus Infection in the Ovary Induces a Continuously Elevated Progesterone Level and Compromises Conception in Interferon Alpha/Beta Receptor-Deficient Mice. J Virol 96:e0118921 (2022). PubMed: 34730391
- Walker RR et al. Glyceollins Trigger Anti-Proliferative Effects in Hormone-Dependent Aromatase-Inhibitor-Resistant Breast Cancer Cells through the Induction of Apoptosis. Int J Mol Sci 23:N/A (2022). PubMed: 35270029
- Fadeyibi O et al. The Role of Lipid Rafts and Membrane Androgen Receptors in Androgen's Neurotoxic Effects. J Endocr Soc 6:bvac030 (2022). PubMed: 35308305
- Chen T et al. Effect of e-cigarette refill liquid on follicular development and estrogen secretion in rats. Tob Induc Dis 20:36 (2022). PubMed: 35529323