Anti-Aromatase antibody (ab35604)
- Product nameAnti-Aromatase antibodySee all Aromatase primary antibodies ...
- DescriptionRabbit polyclonal to Aromatase
- Tested applicationsWB, ICC/IF, IHC-Fr, IHC-P more details
- Species reactivityReacts with: Mouse, Dog, Human
Predicted to work with: Rabbit
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Aromatase.
(Peptide available as ab35661.)
- Positive control
- This antibody gave a positive signal in HL60 whole cell lysate as well as the following tissue lysates: Human placenta; Mouse ovary.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab35604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: 1/50 (see Abreview).
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- FunctionCatalyzes the formation of aromatic C18 estrogens from C19 androgens.
- Tissue specificityBrain, placenta and gonads.
- Involvement in diseaseDefects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries.
- Sequence similaritiesBelongs to the cytochrome P450 family.
- Cellular localizationMembrane.
- ARO 1 antibody
- ARO antibody
- ARO1 antibody
- ARO1 antibody
- Aromatase antibody
- CP19A_HUMAN antibody
- CPV antibody
- CPV1 antibody
- CYAR antibody
- CYP19 antibody
- CYP19A1 antibody
- CYPXIX antibody
- Cytochrome P-450AROM antibody
- Cytochrome P450 19A1 antibody
- Cytochrome P450 family 19 subfamily A polypeptide 1 antibody
- Cytochrome P450, family 19, subfamily A, polypeptide 1 antibody
- Cytochrome P450, subfamily XIX (aromatization of androgens) antibody
- Estrogen synthase antibody
- Estrogen synthetase antibody
- Flavoprotein linked monooxygenase antibody
- MGC104309 antibody
- Microsomal monooxygenase antibody
- OTTHUMP00000162543 antibody
- OTTHUMP00000198350 antibody
- P 450AROM antibody
- P450AROM antibody
Anti-Aromatase antibody images
ab35604 staining Aromatase in dog ovary tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in acetone for 10 minutes and blocked in TBS buffer (1%BSA + 10%NGS) for 2 hours at 25°C. The primary antibody was diluted 1/50 and incubated with sample for 16 hours at 4°C. A HRP conjugated goat polyclonal to rabbit IgG (H&L), diluted 1/100 was used as secondary.
ICC/IF image of ab35604 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab35604, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa cells at 5µg/ml.
All lanes : Anti-Aromatase antibody (ab35604) at 1 µg/ml
Lane 1 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745) - Blocked with 5% BSA
Lane 2 : Ovary (Mouse) Tissue Lysate - Blocked with 5% BSA
Lane 3 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate - Blocked with 5% BSA
Lane 4 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745) - Blocked with 3% Milk
Lane 5 : Ovary (Mouse) Tissue Lysate - Blocked with 3% Milk
Lane 6 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate - Blocked with 3% Milk
Lysates/proteins at 10 µg per lane.
Lanes 1, 2, 4, 5 & 6 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Lane 3 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 5000 µg/ml
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 58 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Exposure time : 90 secondsBovine serum albumin (BSA -5%) was used as the blocking agent in lanes 1-3 where as Milk (3%) was used as the blocking agent in lanes 4-6. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
References for Anti-Aromatase antibody (ab35604)
This product has been referenced in:
- Gatson JW et al. Aromatase is increased in astrocytes in the presence of elevated pressure. Endocrinology 152:207-13 (2011). WB . Read more (PubMed: 21047944) »
- Charitidi K & Canlon B Estrogen receptors in the central auditory system of male and female mice. Neuroscience 165:923-33 (2010). Read more (PubMed: 19925852) »