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Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Aromatase.
(Peptide available as ab35661.)
Our Abpromise guarantee covers the use of ab35604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).Can be blocked with Human Aromatase peptide (ab35661).|
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab35604 staining Aromatase in dog ovary tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in acetone for 10 minutes and blocked in TBS buffer (1%BSA + 10%NGS) for 2 hours at 25°C. The primary antibody was diluted 1/50 and incubated with sample for 16 hours at 4°C. A HRP conjugated goat polyclonal to rabbit IgG (H&L), diluted 1/100 was used as secondary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"