Overview

  • Product nameAnti-Aromatase antibody
    See all Aromatase primary antibodies
  • Description
    Rabbit polyclonal to Aromatase
  • Tested applicationsWB, ICC/IF, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Dog, Human
    Predicted to work with: Rabbit
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Aromatase.

    (Peptide available as ab35661.)

  • Positive control
    • This antibody gave a positive signal in Human placenta tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab35604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).Can be blocked with Human Aromatase peptide (ab35661).
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr 1/50.
IHC-P Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionCatalyzes the formation of aromatic C18 estrogens from C19 androgens.
  • Tissue specificityBrain, placenta and gonads.
  • Involvement in diseaseDefects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
    Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries.
  • Sequence similaritiesBelongs to the cytochrome P450 family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARO 1 antibody
    • ARO antibody
    • ARO1 antibody
    • ARO1 antibody
    • Aromatase antibody
    • CP19A_HUMAN antibody
    • CPV antibody
    • CPV1 antibody
    • CYAR antibody
    • CYP19 antibody
    • CYP19A1 antibody
    • CYPXIX antibody
    • Cytochrome P-450AROM antibody
    • Cytochrome P450 19A1 antibody
    • Cytochrome P450 family 19 subfamily A polypeptide 1 antibody
    • Cytochrome P450, family 19, subfamily A, polypeptide 1 antibody
    • Cytochrome P450, subfamily XIX (aromatization of androgens) antibody
    • Estrogen synthase antibody
    • Estrogen synthetase antibody
    • Flavoprotein linked monooxygenase antibody
    • MGC104309 antibody
    • Microsomal monooxygenase antibody
    • OTTHUMP00000162543 antibody
    • OTTHUMP00000198350 antibody
    • P 450AROM antibody
    • P450AROM antibody
    see all

Anti-Aromatase antibody images

  • Anti-Aromatase antibody (ab35604) at 1 µg/ml + Placenta (Human) Tissue Lysate - adult normal tissue (ab29745) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 150 seconds

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab35604 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • ab35604 staining Aromatase in dog ovary tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in acetone for 10 minutes and blocked in TBS buffer (1%BSA + 10%NGS) for 2 hours at 25°C. The primary antibody was diluted 1/50 and incubated with sample for 16 hours at 4°C. A HRP conjugated goat polyclonal to rabbit IgG (H&L), diluted 1/100 was used as secondary.

    See Abreview

  • ICC/IF image of ab35604 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab35604, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa cells at 5µg/ml.

References for Anti-Aromatase antibody (ab35604)

This product has been referenced in:
  • Laws MJ  et al. Dysregulated estrogen receptor signaling in the hypothalamic-pituitary-ovarian axis leads to ovarian epithelial tumorigenesis in mice. PLoS Genet 10:e1004230 (2014). IHC-P ; Mouse . Read more (PubMed: 24603706) »
  • Dutta S  et al. The steroid hormone environment during primordial follicle formation in perinatal mouse ovaries. Biol Reprod 91:68 (2014). Mouse . Read more (PubMed: 25078683) »

See all 6 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Sample Mouse Cell (Mouse Ovarian Tumor)
Specification Mouse Ovarian Tumor
Permeabilization Yes - 0.1% Triton X
Fixative NBF
Username

Dr. Athi Kannan

Verified customer

Submitted Mar 10 2014

Thanks for your enquiry.

It seems that we have already received an enquiry regarding this same issue in July. A refund was given.

I hope this is helpful. Please contact us again if you need anything further.

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact ...

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Thank you for your reply.

I am sorry to hear that the protocol suggestions did not improve the customer's results. I have found your order and would like to offer you a free of charge replacement antibody or a credit note/refund. Please let m...

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Thank you very much for your interest in our antibodies. For c-Fos, I would recommend either ab7963 or ab87655.  Both of these antibodies will be guaranteed for use in IHC on mouse samples.  For Aromatase, ab18995 and ab35604 will be guaranteed for you...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Dog Tissue sections (ovary)
Specification ovary
Fixative Acetone
Permeabilization Yes - acetone 10 min
Blocking step 1%BSA, 10%NGS in TBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Dr. David Matlack

Verified customer

Submitted Dec 17 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (spleen)
Specification spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Aug 22 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (spleen)
Specification spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization No
Blocking step Sequential peroxidase (5min) and protein (10min) block (prediluted). Reagents from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Aug 19 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"