• Product nameAnti-Aromatase antibodySee all Aromatase primary antibodies ...
  • Description
    Rabbit polyclonal to Aromatase
  • Tested applicationsWB, ICC/IF, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Dog, Human
    Predicted to work with: Rabbit
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Aromatase.

    (Peptide available as ab35661.)

  • Positive control
    • This antibody gave a positive signal in Human placenta tissue lysate.



Our Abpromise guarantee covers the use of ab35604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).Can be blocked with Human Aromatase peptide (ab35661).
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr 1/50.
IHC-P Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • FunctionCatalyzes the formation of aromatic C18 estrogens from C19 androgens.
  • Tissue specificityBrain, placenta and gonads.
  • Involvement in diseaseDefects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
    Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries.
  • Sequence similaritiesBelongs to the cytochrome P450 family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARO 1 antibody
    • ARO antibody
    • ARO1 antibody
    • ARO1 antibody
    • Aromatase antibody
    • CP19A_HUMAN antibody
    • CPV antibody
    • CPV1 antibody
    • CYAR antibody
    • CYP19 antibody
    • CYP19A1 antibody
    • CYPXIX antibody
    • Cytochrome P-450AROM antibody
    • Cytochrome P450 19A1 antibody
    • Cytochrome P450 family 19 subfamily A polypeptide 1 antibody
    • Cytochrome P450, family 19, subfamily A, polypeptide 1 antibody
    • Cytochrome P450, subfamily XIX (aromatization of androgens) antibody
    • Estrogen synthase antibody
    • Estrogen synthetase antibody
    • Flavoprotein linked monooxygenase antibody
    • MGC104309 antibody
    • Microsomal monooxygenase antibody
    • OTTHUMP00000162543 antibody
    • OTTHUMP00000198350 antibody
    • P 450AROM antibody
    • P450AROM antibody
    see all

Anti-Aromatase antibody images

  • Anti-Aromatase antibody (ab35604) at 1 µg/ml + Placenta (Human) Tissue Lysate - adult normal tissue (ab29745) at 20 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    Exposure time : 150 seconds

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab35604 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • ab35604 staining Aromatase in dog ovary tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in acetone for 10 minutes and blocked in TBS buffer (1%BSA + 10%NGS) for 2 hours at 25°C. The primary antibody was diluted 1/50 and incubated with sample for 16 hours at 4°C. A HRP conjugated goat polyclonal to rabbit IgG (H&L), diluted 1/100 was used as secondary.

    See Abreview

  • ICC/IF image of ab35604 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab35604, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa cells at 5µg/ml.

References for Anti-Aromatase antibody (ab35604)

This product has been referenced in:
  • Laws MJ  et al. Dysregulated estrogen receptor signaling in the hypothalamic-pituitary-ovarian axis leads to ovarian epithelial tumorigenesis in mice. PLoS Genet 10:e1004230 (2014). IHC-P ; Mouse . Read more (PubMed: 24603706) »
  • Gatson JW  et al. Aromatase is increased in astrocytes in the presence of elevated pressure. Endocrinology 152:207-13 (2011). WB . Read more (PubMed: 21047944) »

See all 5 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Sample Mouse Cell (Mouse Ovarian Tumor)
Specification Mouse Ovarian Tumor
Permeabilization Yes - 0.1% Triton X
Fixative NBF

Dr. Athi Kannan

Verified customer

Submitted Mar 10 2014

Thanks for your enquiry.

It seems that we have already received an enquiry regarding this same issue in July. A refund was given.

I hope this is helpful. Please contact us again if you need anything further.

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact ...

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Thank you for your reply.

I am sorry to hear that the protocol suggestions did not improve the customer's results. I have found your order and would like to offer you a free of charge replacement antibody or a credit note/refund. Please let m...

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Thank you very much for your interest in our antibodies. For c-Fos, I would recommend either ab7963 or ab87655.  Both of these antibodies will be guaranteed for use in IHC on mouse samples.  For Aromatase, ab18995 and ab35604 will be guaranteed for you...

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Application Immunohistochemistry (Frozen sections)
Sample Dog Tissue sections (ovary)
Specification ovary
Fixative Acetone
Permeabilization Yes - acetone 10 min
Blocking step 1%BSA, 10%NGS in TBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Dr. David Matlack

Verified customer

Submitted Dec 17 2008

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (spleen)
Specification spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted Aug 22 2008

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (spleen)
Specification spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization No
Blocking step Sequential peroxidase (5min) and protein (10min) block (prediluted). Reagents from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted Aug 19 2008