Recombinant
RabMAb

Anti-Aromatase antibody [EPR4534(2)] (ab124776)

Overview

  • Product name
    Anti-Aromatase antibody [EPR4534(2)]
    See all Aromatase primary antibodies
  • Description
    Rabbit monoclonal [EPR4534(2)] to Aromatase
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Rat, Human
    Does not react with: Mouse
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Aromatase aa 1-100. The exact sequence is proprietary.
    (Peptide available as ab186919)

  • Positive control
    • WB: Human placenta, human uterus, rat brain tissue lysate and JAR and NIH:OVCAR 3 cell lysates. IP: Rat brain tissue lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 58 kDa).Can be blocked with Aromatase peptide (ab186919).
IP 1/10 - 1/300.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
    • Tissue specificity
      Brain, placenta and gonads.
    • Involvement in disease
      Defects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
      Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries.
    • Sequence similarities
      Belongs to the cytochrome P450 family.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ARO antibody
      • ARO1 antibody
      • Aromatase antibody
      • CP19A_HUMAN antibody
      • CPV1 antibody
      • CYAR antibody
      • CYP19 antibody
      • Cyp19a1 antibody
      • CYPXIX antibody
      • Cytochrome P-450AROM antibody
      • Cytochrome P450 19A1 antibody
      • Cytochrome P450, family 19, subfamily A, polypeptide 1 antibody
      • Cytochrome P450, subfamily XIX (aromatization of androgens) antibody
      • Estrogen synthase antibody
      • Estrogen synthetase antibody
      • Flavoprotein linked monooxygenase antibody
      • MGC104309 antibody
      • Microsomal monooxygenase antibody
      • OTTHUMP00000162543 antibody
      • OTTHUMP00000198350 antibody
      • P 450AROM antibody
      see all

    Images

    • Anti-Aromatase antibody [EPR4534(2)] (ab124776) at 1/2000 dilution (purified) + Rat brain tissue lysate at 20 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/5000 dilution

      Predicted band size : 58 kDa
      Observed band size : 53 kDa (why is the actual band size different from the predicted?)
      Blocking and dilution buffer: 5% NFDM/TBST
    • Anti-Aromatase antibody [EPR4534(2)] (ab124776) at 1/2000 dilution (purified) + JAR whole cell lysate at 20 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/5000 dilution

      Predicted band size : 58 kDa
      Observed band size : 53 kDa (why is the actual band size different from the predicted?)
      Blocking and dilution buffer: 5% NFDM/TBST
    • ab124776 (purified) at a dilution of 1/300 immunoprecipitating Aromatase in rat brain tissue lysate.

      Lane 1 (input): Rat brain tissue lysate (10µg)

      Lane 2 (+): ab124776 + rat brain tissue lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124776 in rat brain tissue lysate.

      For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/1000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-Aromatase antibody [EPR4534(2)] (ab124776) at 1/1000 dilution (unpurified)

      Lane 1 : Human placenta lysate
      Lane 2 : Human uterus lysate
      Lane 3 : JAR cell lysate
      Lane 4 : NIH:OVCAR 3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 58 kDa
      Observed band size : 53 kDa (why is the actual band size different from the predicted?)
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Peterson A  et al. Exemestane potency is unchanged by common nonsynonymous polymorphisms in CYP19A1: results of a novel anti-aromatase activity assay examining exemestane and its derivatives. Pharmacol Res Perspect 5:e00313 (2017). Read more (PubMed: 28603632) »
    • Ren J  et al. Estrogen upregulates MICA/B expression in human non-small cell lung cancer through the regulation of ADAM17. Cell Mol Immunol N/A:N/A (2014). Read more (PubMed: 25363527) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Hamster Cell lysate - whole cell (BHK17)
    Gel Running Conditions
    Reduced Denaturing (Gel 10%)
    Loading amount
    10 µg
    Specification
    BHK17
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Simon Boudreault

    Verified customer

    Submitted Mar 17 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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