RabMAb

Anti-Aromatase antibody [EPR4534(2)] (ab124776)

Overview

  • Product nameAnti-Aromatase antibody [EPR4534(2)]See all Aromatase primary antibodies ...
  • Description
    Rabbit monoclonal [EPR4534(2)] to Aromatase
  • Tested applicationsWB, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human

    Does not react with

    Mouse
  • Immunogen

    Synthetic peptide corresponding to residues in Aromatase (Human)

  • Positive control
    • WB: Human placenta, Human uterus, JAR or NIH:OVCAR 3 lysates Flow Cyt: Permeabilized NIH:OVCAR 3 cells
  • General notes

    Produced under U.S. Patent No. 5,675,063. A 40 μl trial size is available to purchase for this antibody.

Applications

Our Abpromise guarantee covers the use of ab124776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 58 kDa).Can be blocked with Aromatase peptide (ab186919).
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.
  • Application notesIs unsuitable for ICC/IF or IHC-P.
  • Target

    • FunctionCatalyzes the formation of aromatic C18 estrogens from C19 androgens.
    • Tissue specificityBrain, placenta and gonads.
    • Involvement in diseaseDefects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
      Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries.
    • Sequence similaritiesBelongs to the cytochrome P450 family.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ARO antibody
      • ARO1 antibody
      • Aromatase antibody
      • CP19A_HUMAN antibody
      • CPV1 antibody
      • CYAR antibody
      • CYP19 antibody
      • Cyp19a1 antibody
      • CYPXIX antibody
      • Cytochrome P-450AROM antibody
      • Cytochrome P450 19A1 antibody
      • Cytochrome P450, family 19, subfamily A, polypeptide 1 antibody
      • Cytochrome P450, subfamily XIX (aromatization of androgens) antibody
      • Estrogen synthase antibody
      • Estrogen synthetase antibody
      • Flavoprotein linked monooxygenase antibody
      • MGC104309 antibody
      • Microsomal monooxygenase antibody
      • OTTHUMP00000162543 antibody
      • OTTHUMP00000198350 antibody
      • P 450AROM antibody
      see all

    Anti-Aromatase antibody [EPR4534(2)] images

    • All lanes : Anti-Aromatase antibody [EPR4534(2)] (ab124776) at 1/1000 dilution

      Lane 1 : Human placenta lysate
      Lane 2 : Human uterus lysate
      Lane 3 : JAR lysate
      Lane 4 : NIH:OVCAR 3 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size : 58 kDa
      Observed band size : 53 kDa (why is the actual band size different from the predicted?)
    • ab124776 (red), at 1/100, staining Arotamase in permeabilized NIH:OVCAR 3 cells. Rabbit IgG was used as a negative control (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Aromatase antibody [EPR4534(2)] (ab124776)

    ab124776 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"