• NatureSynthetic

Associated products


Our Abpromise guarantee covers the use of ab51924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Aromatase antibody (ab18995)

  • Purity> 90 % SDS-PAGE.

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: 0.02% Thimerosal (merthiolate)
    Constituents: 0.1% BSA, PBS, pH 7.2

General Info

  • Alternative names
    • ARO
    • ARO1
    • Aromatase
    • CP19A_HUMAN
    • CPV1
    • CYAR
    • CYP19
    • Cyp19a1
    • CYPXIX
    • Cytochrome P-450AROM
    • Cytochrome P450 19A1
    • Cytochrome P450, family 19, subfamily A, polypeptide 1
    • Cytochrome P450, subfamily XIX (aromatization of androgens)
    • Estrogen synthase
    • Estrogen synthetase
    • Flavoprotein linked monooxygenase
    • MGC104309
    • Microsomal monooxygenase
    • OTTHUMP00000162543
    • OTTHUMP00000198350
    • P 450AROM
    see all
  • FunctionCatalyzes the formation of aromatic C18 estrogens from C19 androgens.
  • Tissue specificityBrain, placenta and gonads.
  • Involvement in diseaseDefects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
    Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries.
  • Sequence similaritiesBelongs to the cytochrome P450 family.
  • Cellular localizationMembrane.
  • Information by UniProt

References for Aromatase peptide (ab51924)

ab51924 has not yet been referenced specifically in any publications.

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Application Western blot

Review text: How long did you incubate the protein and antibody mix before adding it to the blot?
First Time: Incubation time of antibody and protein (peptide): two hour at room temperature
Second Time:Incubation time of antibody and protein (peptide): One hour at 37 degree C
How much antibody/protein mix was added to the blot?
Antibody used for the reaction is 1ug and protein (peptide) 5ug (1:5 ratio)
Sample: Mouse Tissue lysate - other (BRAIN)
Loading amount: 15 µg
Specification: BRAIN
Blocking step: Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details
Concentration: 5 µg/ml
Incubation time: 1 hour(s) and 0 minute(s) · Temperature: 30°C

Primary antibody (in addition to 'Aromatase peptide')
Concentration: 1 µg/ml

Secondary antibody
Name: Non-Abcam antibody was used: anti - rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/20000

Detection method: ECL
Exposure: 2 minute(s) and 0 second(s)
Bands: Specific: 54 kDa Non-specific: 104, 77, 71, 67, 46, 36, kDa

Additional data
Peptide usage 5 times - 1ug of antibody and 5ug of blocking peptide.
Abcam response: The blocking peptide was the same as the immunogen. At the moment we have not found a reason why blocking is not taking place,
We have not had similar complaints for Aromatase peptide (ab51924).
More blocking peptide might be needed in customer's case.

Abcam user community

Verified customer

Submitted Apr 28 2011