Overview

  • Product nameAnti-ARP10 antibody
    See all ARP10 primary antibodies
  • Description
    Mouse polyclonal to ARP10
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-182 of Human ARP10

  • Positive control
    • Human colon tissue lysate and transfected 293 cell line lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab73055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Expected band of 21.5 kDa (Isoform 2). Detects a band of approximately 19/20 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceARP10 belongs to the cytidine and deoxycytidylate deaminase family. It is a poorly expressed deaminase that is ineffective at inhibiting retroviral replication. ARP10 has DNA deaminase (cytidine deaminase) activity but is not able to mediate G-to-A hypermutation in newly synthesized retroviral DNA. It is present at low level in 293T cells. It is considered an unstable protein, suggesting that it may be degraded by the proteasome (at protein level). Weakly expressed in testis, ovary, fetal liver and skin. There are two named isoforms. ARP10 from old world monkeys has retained its antiviral activity, while it is lost in other primates. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22.
    • Cellular localizationNucleus. Cytoplasm. Note: Mostly nuclear.
    • Database links
    • Alternative names
      • A3H antibody
      • APOBEC related protein 10 antibody
      • APOBEC3H antibody
      • Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3H antibody
      • ARP 10 antibody
      • DNA dC->dU-editing enzyme APOBEC-3H antibody
      see all

    Anti-ARP10 antibody images

    • Anti-ARP10 antibody (ab73055) at 1/500 dilution + human colon tissue lysate at 25 µg

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-ARP10 antibody (ab73055) at 1/500 dilution

      Lane 1 : ARP10 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Observed band size : 19 kDa (why is the actual band size different from the predicted?)

    References for Anti-ARP10 antibody (ab73055)

    This product has been referenced in:

    See 1 Publication for this product

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