Anti-Arp2 antibody (ab226476)
Key features and details
- Rabbit polyclonal to Arp2
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Arp2 antibody
See all Arp2 primary antibodies -
Description
Rabbit polyclonal to Arp2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Zebrafish, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide within Human Arp2 aa 25-75. The exact sequence is proprietary.
Database link: P61160 -
Positive control
- WB: HeLa, HEK-293T, Jurkat and NIH/3T3 cell lysate. IHC: Human skin squamous carcinoma tissue. IP: HEK-293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 45 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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IHC-P |
1/1000 - 1/5000.
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 45 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
IHC-P
1/1000 - 1/5000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Target
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Function
Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. -
Sequence similarities
Belongs to the actin family. ARP2 subfamily. -
Cellular localization
Cytoplasm > cytoskeleton. Cell projection. - Information by UniProt
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Database links
- Entrez Gene: 396147 Chicken
- Entrez Gene: 538486 Cow
- Entrez Gene: 10097 Human
- Entrez Gene: 66713 Mouse
- Entrez Gene: 100173969 Orangutan
- Entrez Gene: 289820 Rat
- Entrez Gene: 443741 Xenopus laevis
- Entrez Gene: 406820 Zebrafish
see all -
Alternative names
- Actin like protein 2 antibody
- Actin related protein 2 antibody
- Actin-like protein 2 antibody
see all
Images
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All lanes : Anti-Arp2 antibody (ab226476) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Exposure time: 10 seconds -
Arp2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN buffer; 1.0 mg for IP, 20% of IP loaded) with ab226476 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226476 at 1 µg/ml.
Lane 1: ab226476 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
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Human skin squamous carcinoma tissue stained for Arp2 using ab226476 at 1/5000 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226476 has not yet been referenced specifically in any publications.