Overview

  • Product nameAnti-Arp2 antibody [FMS96]
    See all Arp2 primary antibodies
  • Description
    Mouse monoclonal [FMS96] to Arp2
  • SpecificityThis antibody recognizes Arp2 (approx. 40-45 kDa).
  • Tested applicationsSuitable for: Indirect ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • Total cell extract of C2 mouse myoblast cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab49674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 40-45 kDa (predicted molecular weight: 45 kDa).

Target

  • FunctionFunctions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament.
  • Sequence similaritiesBelongs to the actin family. ARP2 subfamily.
  • Cellular localizationCytoplasm > cytoskeleton. Cell projection.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin like protein 2 antibody
    • Actin related protein 2 antibody
    • Actin-like protein 2 antibody
    • Actin-related protein 2 antibody
    • ACTR2 antibody
    • ARP2 actin related protein 2 homolog (yeast) antibody
    • ARP2 antibody
    • ARP2/3 COMPLEX antibody
    • ARP2_HUMAN antibody
    • OTTHUMP00000159937 antibody
    • OTTHUMP00000202270 antibody
    see all

Anti-Arp2 antibody [FMS96] images

  • C2C12 cells were incubated with ab49674 at 10 µg/ml followed by Goat Anti Mouse IgG Alexa-488 (green staining). The red staining is obtained by staining actin with Alexa-546 phalloidin.
  • Immunofluorescence analysis of dendritic cells infected with HIV, staining Arp2 (blue) with ab49674 at 5 mg/ml.

References for Anti-Arp2 antibody [FMS96] (ab49674)

This product has been referenced in:
  • Wang F  et al. Arp2/3 complex inhibition prevents meiotic maturation in porcine oocytes. PLoS One 9:e87700 (2014). ICC/IF ; Pig . Read more (PubMed: 24498171) »
  • Naj X  et al. The formins FMNL1 and mDia1 regulate coiling phagocytosis of Borrelia burgdorferi by primary human macrophages. Infect Immun 81:1683-95 (2013). Human . Read more (PubMed: 23460512) »

See all 6 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"