Anti-ARP2/3 subunit 1B antibody (ab99314)

Overview

  • Product nameAnti-ARP2/3 subunit 1B antibody
    See all ARP2/3 subunit 1B primary antibodies
  • Description
    Rabbit polyclonal to ARP2/3 subunit 1B
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 272 and 322 of Human ARP2/3 subunit 1B (NP_005711.1).

  • Positive control
    • HeLa, 293T and NIH3T3 cell lysates

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab99314 was affinity purified using an epitope specific to ARP2/3 Subunit 1B immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

Anti-ARP2/3 subunit 1B antibody images

  • ab99314 at 1 µg/ml detecting ARP2/3 subunit 1B in HeLa whole cell lysate by WB following IP.
    Lane 1: ab99314 at 3µg/mg of lysate
    Lane 2: IP with an antibody which recognizes an downstream epitope of ARP2/3 subunit 1B
    Lane 3: control IgG.
    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
    Detection: Chemiluminescence with an exposure time of 3 seconds.
  • All lanes : Anti-ARP2/3 subunit 1B antibody (ab99314) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg
    Lane 5 : NIH3T3 cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 41 kDa


    Exposure time : 3 minutes

References for Anti-ARP2/3 subunit 1B antibody (ab99314)

ab99314 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Testis)
Permeabilization Yes - 2% Triton X 100
Specification Testis
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Jul 06 2016

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Rat Tissue lysate - whole (Testis)
Specification Testis
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 05 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"