Overview

  • Product name
    Anti-Arp3 antibody [FMS338]
    See all Arp3 primary antibodies
  • Description
    Mouse monoclonal [FMS338] to Arp3
  • Tested applications
    Suitable for: Indirect ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human, African green monkey
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • Whole cell lysate of mouse myoblast C2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab49671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.

Array: Use at an assay dependent dilution.

ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 40-45 kDa (predicted molecular weight: 47 kDa).

Target

  • Relevance
    The Arp2/3 complex involved in Golgi polerization in NIH3T3 cells. In a different model it has been shown that the Arp2/3 complex is necessary for neutrophil chemotaxis and phagocytosis.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • Actin like protein 3 antibody
    • Actin related protein 3 antibody
    • ACTR3 antibody
    • ARP3 actin related protein 3 homolog (yeast) antibody

Anti-Arp3 antibody [FMS338] images

  • C2C12 cells were incubated with ab49671 at 10µg/ml followed by Alexa-488 conjugated secondary antibody(green staining). The red stain is obtained by staining actin with Alexa-456 phalloidin.

References for Anti-Arp3 antibody [FMS338] (ab49671)

This product has been referenced in:
  • Liu Y  et al. LMO2 Enhances Lamellipodia/Filopodia Formation in Basal-Type Breast Cancer Cells by Mediating ARP3-Profilin1 Interaction. Med Sci Monit 23:695-703 (2017). Read more (PubMed: 28170369) »
  • Yan Y  et al. Augmented AMPK activity inhibits cell migration by phosphorylating the novel substrate Pdlim5. Nat Commun 6:6137 (2015). Read more (PubMed: 25635515) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
African Green Monkey Cell (fibroblasts like cells)
Specification
fibroblasts like cells
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Mr. Stephane Gross

Verified customer

Submitted Mar 26 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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