Overview

  • Product name
    Anti-Arp5 antibody - ChIP Grade
  • Description
    Rabbit polyclonal to Arp5 - ChIP Grade
  • Tested applications
    Suitable for: WB, ChIPmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant full length protein Arp 5.

  • Positive control
    • S. cereviseae lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab12099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
ChIP Use at an assay dependent concentration.

Target

  • Relevance
    The budding yeast INO80 complex is a conserved ATP-dependent nucleosome remodeler containing actin-related proteins Arp5 and Arp8. Strains lacking INO80, ARP5, or ARP8 have defects in transcription. Ino80, Arp5, and Arp8 are recruited to an HO-induced DSB, where a phosphorylated form of H2A accumulates. Recruitment of Ino80 is compromised in cells lacking the H2A phosphoacceptor S129. INO80-mediated chromatin remodeling seems to occur directly at DSBs, where it appears to facilitate processing of the lesion.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • Actin-like protein ARP5 antibody
      • ARP5_YEAST antibody
      • P53946 antibody

    Anti-Arp5 antibody - ChIP Grade images

    • Anti-Arp5 antibody - ChIP Grade (ab12099) + S.cerevisiae lysate

      Secondary
      Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution

      Predicted band size : 87 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 70 kDa (possible cross reactivity).
    • All lanes : Anti-Arp5 antibody - ChIP Grade (ab12099) at 0.36 µg/ml

      Lane 1 : S. cerevisiae whole cell lysate (wild type)
      Lane 2 : S. cerevisiae whole cell lysate (arp5 knockout)
      Lane 3 : S. cerevisiae whole cell lysate (arp8 knockout)
      Lane 4 : S. cerevisiae whole cell lysate (ino80 knockout)
      Lane 5 : S. cerevisiae whole cell lysate (anc1 knockout)
      Lane 6 : S. cerevisiae whole cell lysate (nhp10 knockout)
      Lane 7 : S. cerevisiae whole cell lysate (wild type, treated with MMS)
      Lane 8 : S. cerevisiae whole cell lysate (arp8 knockout, treated with MMS)


      Predicted band size : 87 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)

    References for Anti-Arp5 antibody - ChIP Grade (ab12099)

    This product has been referenced in:
    • Panday A  et al. Yeast high mobility group protein HMO1 stabilizes chromatin and is evicted during repair of DNA double strand breaks. Nucleic Acids Res 43:5759-70 (2015). ChIP . Read more (PubMed: 25979266) »
    • Xue Y  et al. The Ino80 complex prevents invasion of euchromatin into silent chromatin. Genes Dev 29:350-5 (2015). CHIPseq . Read more (PubMed: 25691465) »

    See all 3 Publications for this product

    Product Wall

    Application
    ChIP
    Detection step
    Other
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (Whole cell lysate)
    Specification
    Whole cell lysate
    Negative control
    No Antibody sample
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 20 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 37% Formaldehyde
    Positive control
    Sample after 1 hour of DNA double strand break
    Username

    Mr. Arvind Panday

    Verified customer

    Submitted Jul 09 2014

    Application
    Western blot
    Loading amount
    3.75e+006 cells
    Gel Running Conditions
    Reduced Denaturing (NUPAGE precasted Invitrogen 10% SDS-PAGE)
    Sample
    Schizosaccharomyces pombe Cell lysate - whole cell (whole cell extract)
    Specification
    whole cell extract
    Blocking step
    0.5% BSA + 5% non fat milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 17 2013

    Thank you for contacting us. I have received the following information from the lab: Ab12099 (ARP5): Unfortunately, the full WB image showing the knock-out experiment is not on file, thus we are not able to determine if there were a...

    Read More

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